Dopaminergic differentiation of human pluripotent cells.
Dopaminergic differentiation of human pluripotent cells.
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DOI：
10.1002/9780470151808.sc01h06s22
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发表时间：
2012-08-01
影响因子：
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通讯作者：
Gage, Fred H
中科院分区：
文献类型：
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作者：
Boyer, Leah F;Campbell, Benjamin;Gage, Fred H
在这里，我们描述了多巴胺能分化多能干细胞的方案。我们已经优化和比较了两种不同的方案，这两种方案都是化学定义的，都适用于胚胎和诱导的多能干细胞。首先，我们提出了一个基于玫瑰花环形成的五步法(基本协议1)；然后我们描述了一个基于抑制交替发育途径的单层范式(基本协议2)。将多能细胞定向分化为特定类型的细胞是了解人类发育和实现这些细胞在生物医学上的相关性的关键一步，无论是用于替代治疗还是疾病建模。
Here we describe protocols for the dopaminergic differentiation of pluripotent stem cells. We have optimized and compared two distinct protocols, both of which are chemically defined and applicable to both embryonic and induced pluripotent stem cells. First, we present a five-step method based on rosette formation (Basic Protocol 1); then we describe a monolayer paradigm based on inhibition of alternate developmental pathways (Basic Protocol 2). Directed differentiation of pluripotent cells into specific cell types is a crucial step towards understanding human development and realizing the biomedical relevance of these cells, whether for replacement therapy or disease modeling.