Genetic diversity of Neisseria gonorrhoeae housekeeping genes.
Genetic diversity of Neisseria gonorrhoeae housekeeping genes.
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淋病奈瑟氏菌管家基因的遗传多样性。
DOI：
10.1128/jcm.41.1.197-204.2003
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发表时间：
2003
影响因子：
9.4
通讯作者：
Demma,JamesC
中科院分区：
文献类型：
--
作者：
Viscidi,RaphaelP;Demma,JamesC
淋球菌的分子分型是淋球菌感染和传播流行病学研究的重要工具。最近发展起来的多位点序列分型（MLST）方法是基于管家基因之间的遗传变异。作为这样一种方法的发展的初步调查，我们的特点是在18个淋球菌管家基因位点的遗传多样性。从24个分离株中测序了大约17，500个核苷酸，跨越18个位点。包括已完全测序的菌株FA 1090和GenBank中3个独特的glnA序列，18个位点的等位基因数为2 - 18，平均每个位点8.3个等位基因。大多数多态性位点沿着基因随机分布，与DNA序列的点突变进化一致。此外，还检测到了几个簇集突变和插入或缺失的实例，这些突变和插入或缺失最有可能通过重组事件发生。虽然净化选择是主导力量，推动这些管家基因的进化，积极的选择也出现在abcZandgpdh基因座。25个完全表征的菌株每个都具有独特的等位基因谱，只有三个位点（pilA，abcZ和piporpgi 2）。基于持家基因等位基因谱的分子分型比核苷酸测序或茶碱基因分型更好地解决了分离株。16名性接触者的pilA、abcZ和serC基因座的等位基因谱完全相同。一个潜在的MLST为N。根据7个管家基因座的约500- 600-bp的基因片段，淋病将包括pilA、abcZ、serC、glnA、gdh、gnd和piploci。
Molecular typing ofNeisseria gonorrhoeaestrains is an important tool for epidemiological studies of gonococcal infection and transmission. The recently developed multilocus sequence typing (MLST) method is based on the genetic variation among housekeeping genes. As a preliminary investigation for the development of such a method, we characterized the genetic diversity at 18 gonococcal housekeeping gene loci. Approximately 17,500 nucleotides, spanning 18 loci, were sequenced from 24 isolates. Including strain FA 1090, which has been fully sequenced, and three uniqueglnAsequences from GenBank, the number of alleles identified for the 18 loci ranged from 2 to 18, with a mean of 8.3 alleles per locus. The majority of polymorphic sites were distributed randomly along the genes, consistent with evolution of DNA sequences by point mutation. In addition, several examples of clustered mutations and insertions or deletions were detected, which most likely occurred by recombinational events. While purifying selection is the dominant force driving the evolution of these housekeeping genes, positive selection also appeared to operate on theabcZandgpdhloci. The 25 completely characterized strains each had a unique allelic profile with as few as three loci (pilA,abcZ, andpiporpgi2). Molecular typing based on the allelic profile of housekeeping genes resolved the isolates better than eitherporBnucleotide sequencing or typing of theopagene. The allelic profiles for thepilA,abcZ, andserCloci of paired strains from 16 sexual contacts were identical. A potential MLST forN. gonorrhoeae, based on ∼500- to 600-bp gene fragments of seven housekeeping gene loci, would include thepilA,abcZ,serC,glnA,gdh,gnd, andpiploci.