Enzymatic production and characterization of manno-oligosaccharides from Gleditsia sinensis galactomannan gum
Enzymatic production and characterization of manno-oligosaccharides from Gleditsia sinensis galactomannan gum
复制标题
皂角半乳甘露聚糖胶的酶法生产及甘露寡糖的表征
DOI：
10.1016/j.ijbiomac.2013.01.025
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发表时间：
2013-04-01
影响因子：
8.2
通讯作者：
Jiang, Jian-Xin
中科院分区：
文献类型：
--
作者：
Jian, Hong-Lei;Zhu, Li-Wei;Jiang, Jian-Xin
Enzymatic hydrolysis of Gleditsia sinensis gum was performed to produce manno-oligosaccharides having functional applications as dietary fiber and prebiotics. The optimum hydrolysis conditions, including enzyme loading, temperature and time, from response surface methodology were 8.1 U/g, 57.4 degrees C and 34.1 h, respectively. The yield of DP 1-5 oligosaccharides was 75.9% (29.1 g/L). The Michaelis-Menten kinetics and molecular weight distribution were determined. The obtained oligosaccharides were further separated by HPLC and SEC, and the galactose distribution of G. sinensis gum was elucidated. Results indicated that G. sinensis gum has potential to produce value-added oligosaccharides in food industries. (C) 2013 Elsevier B.V. All rights reserved.
