Preparation of surface-imprinted microspheres using ionic liquids as novel cross-linker for recognizing an immunostimulating peptide
Preparation of surface-imprinted microspheres using ionic liquids as novel cross-linker for recognizing an immunostimulating peptide
复制标题
使用离子液体作为识别免疫刺激肽的新型交联剂制备表面印迹微球
DOI：
10.1007/s10853-017-1005-x
复制
发表时间：
2017-07-01
影响因子：
4.5
通讯作者：
Du, Chunbao
中科院分区：
文献类型：
--
作者：
Ding, Shichao;Hu, Xiaoling;Du, Chunbao
最近，人们积极研究利用仿生方法设计和制备新型功能材料，以实现生物分子的高吸附和精确选择性捕获。在这项工作中，通过在室温下水介质中表面引发-引发聚合反应成功合成了具有锚定在SiO2核颗粒上的均质聚合物壳的分子印迹微球（MIM）。在此，选择来自人酪蛋白的临床相关免疫刺激六肽（IHHC）作为模板。此外，合成了一种新型的具有多个结合位点的双咪唑鎓双离子液体，并将其用作交联剂，以提高MIM的识别性能并同时增强其吸附能力。通过各种方法对所制备的微球进行了表征。结果表明，MIM不仅对IHHC表现出优异的选择性识别能力，而且通过定向和非定向相互作用的协同效应，具有高吸附能力，这证明了其在实际应用中的潜在用途。
Recently, the design and preparation of novel functional materials using biomimetic approaches for the high adsorption and precise selective capture of biomolecules have been actively investigated. In this work, molecularly imprinted microspheres (MIMs) with a homogeneous polymer shell anchored onto SiO2core particles were successfully synthesized via the surface iniferter-initiated polymerization under room temperature in aqueous media. Herein, a clinically relevant immunostimulating hexapeptide from human casein (IHHC) was chosen as a template. Moreover, a novel double imidazolium dicationic ionic liquid with multiple binding sites was synthesized and used as the cross-linker to improve the recognition performance and enhance the adsorption capacity of MIMs simultaneously. The as-prepared microspheres were characterized through various methods. Results revealed that MIMs not only exhibited excellent selective recognition for IHHC but also possessed a high adsorption capacity through the synergistic effect of directional and non-directional interactions, which demonstrated their potential use in the practical application.