EXPRESSION OF 4 MYOSIN HEAVY-CHAIN ISOFORMS WITH DEVELOPMENT IN MOUSE UTERUS
EXPRESSION OF 4 MYOSIN HEAVY-CHAIN ISOFORMS WITH DEVELOPMENT IN MOUSE UTERUS
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DOI：
10.1002/cm.970250406
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发表时间：
1993-01-01
影响因子：
--
通讯作者：
WOLF, JA
中科院分区：
文献类型：
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作者：
EDDINGER, TJ;WOLF, JA
在平滑肌组织中，已报道了两种或三种肌球蛋白重链（MHC）亚型（SM 1、SM 2和/或NM）。在小鼠子宫组织中，在高分辨率SDS聚丙烯酰胺凝胶上可以分辨MHC区域中的四条带。使用平滑肌（SM）MHC特异性和非肌肉（NM）MHC特异性多克隆抗体进行的Western印迹显示，MHC区域的上两条带是SM亚型，而下两条带是NM亚型。这四条带的一维肽图谱显示，在α-糜蛋白酶消化后，每条带都具有独特的多肽片段模式。用组织匀浆测定了小鼠子宫、主动脉、膀胱和胃（6个年龄，10-150天）中肌球蛋白重链（MHC）的发育表达。在子宫中，SM MHC的相对含量随着年龄的增加而增加，而NM MHC的相对含量随着年龄的增加而减少。从第10天到第30天，小鼠主动脉显示SM MHC的显著增加和NM MHC的显著降低，这与大鼠主动脉报告的数据相似。虽然膀胱和胃都含有相对少量的NM MHC（约10%或更少），但这些数量确实随着发育而减少。在整个发育过程中，子宫的SM 1：SM 2比率保持较高（150天时为3.4）;主动脉、膀胱和胃也开始较高，但在150天的动物中趋于1.0。在子宫中存在四种MHC亚型，具有独特的发育调控表达，这与这些亚型的独特功能作用的假设一致。 (C)1993 Wiley-Liss，Inc.
In smooth muscle tissue, two or three isoforms of myosin heavy chain (MHC) have been reported (SM1, SM2, and/or NM). In mouse uterus tissue, four bands in the region of the MHC's can be resolved on high resolution SDS polyacrylamide gels. Western blots using smooth muscle (SM) MHC-specific and nonmuscle (NM) MHC-specific polyclonal antibodies show the upper two bands in the MHC region are SM isoforms, whereas the lower two bands are NM isoforms. One-dimensional peptide maps of these four bands show each to have a unique pattern of polypeptide fragments following alpha-chymotrypsin digestion. Developmental expression of myosin heavy chains (MHC) in mouse uterus, aorta, bladder, and stomach (6 ages, 10-150 days) was determined using tissue homogenates. In the uterus, both SM MHC's show an increase in relative content with increasing age, whereas the NM MHC's show a decrease. The mouse aorta shows a significant increase in the SM MHC's and a significant decrease in the NM MHC from day 10 to day 30, which is similar to data reported for the rat aorta. Whereas both the bladder and stomach contain relatively small amounts of NM MHC's (approximately 10% or less), these quantities do show decreases with development. The SM1:SM2 ratio for the uterus remains high (3.4 at 150 days) through development; the aorta, bladder, and stomach also start out high, but tend toward 1.0 in the 150-day animals. The presence of four MHC isoforms in the uterus with unique developmental regulation of expression is consistent with hypotheses of unique functional roles for these isoforms. (C) 1993 Wiley-Liss, Inc.