INDUCTION OF PSEUDOHYPHAL GROWTH BY OVEREXPRESSION OF PHD1, A SACCHAROMYCES-CEREVISIAE GENE-RELATED TO TRANSCRIPTIONAL REGULATORS OF FUNGAL DEVELOPMENT
INDUCTION OF PSEUDOHYPHAL GROWTH BY OVEREXPRESSION OF PHD1, A SACCHAROMYCES-CEREVISIAE GENE-RELATED TO TRANSCRIPTIONAL REGULATORS OF FUNGAL DEVELOPMENT
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DOI：
10.1128/mcb.14.3.2100
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发表时间：
1994-03-01
影响因子：
5.3
通讯作者：
FINK, GR
中科院分区：
文献类型：
--
作者：
GIMENO, CJ;FINK, GR
当缺乏氮时，出芽酵母酿酒酵母的MATa/MAT α细胞经历二态过渡到假菌丝生长。建立了酿酒酵母假菌丝生长突变体的视觉遗传筛选系统，称为PHD （pseudo菌丝决定因素）。利用PHD筛选鉴定了7个酿酒酵母基因，这些基因在氮饥饿培养基上生长的MATa/MAT α细胞中过表达可导致假菌丝早熟和异常旺盛的生长。研究了PHD1基因在富营养培养基上的过表达诱导侵袭性假菌丝生长。PHD1映射到XI染色体，预计编码366个氨基酸的蛋白质。PHD1具有SW14-和mbp1 -样的DNA结合基序，在100多个氨基酸中与细粒曲霉（Aspergillus nidulans StuA）区域相同73%。在分生孢子形态发生过程中，StuA调控两次假菌丝生长样细胞分裂。表位标记的PHD1通过间接免疫荧光定位到细胞核。这些事实表明PHD1可能作为转录调节蛋白起作用。PHD1在野生型单倍体菌株中过表达不会诱导假菌丝生长。有趣的是，由于BUD4基因突变，PHD1过表达增强了具有二倍体极性出芽模式的单倍体菌株的假菌丝生长。此外，缺乏PHD1的野生型二倍体菌株在缺乏氮的情况下会产生假菌丝生长。本文讨论了PHD1在假菌丝生长中的可能功能，以及利用PHD筛选从异源生物中鉴定形态发生调控基因。
When starved for nitrogen, MATa/MAT alpha cells of the budding yeast Saccharomyces cerevisiae undergo a dimorphic transition to pseudohyphal growth. A visual genetic screen, called PHD (pseudohyphal determinant), for S. cerevisiae pseudohyphal growth mutants was developed. The PHD screen was used to identify seven S. cerevisiae genes that when overexpressed in MATa/MAT alpha cells growing on nitrogen starvation medium cause precocious and unusually vigorous pseudohyphal growth. PHD1, a gene whose overexpression induced invasive pseudohyphal growth on a nutritionally rich medium, was characterized. PHD1 maps to chromosome XI and is predicted to encode a 366-amino-acid protein. PHD1 has a SW14- and MBP1-like DNA binding motif that is 73% identical over 100 amino acids to a region of Aspergillus nidulans StuA. StuA regulates two pseudohyphal growth-like cell divisions during conidiophore morphogenesis. Epitope-tagged PHD1 was localized to the nucleus by indirect immunofluorescence. These facts suggest that PHD1 may function as a transcriptional regulatory protein. Overexpression of PHD1 in wild-type haploid strains does not induce pseudohyphal growth. Interestingly, PHD1 overexpression enhances pseudohyphal growth in a haploid strain that has the diploid polar budding pattern because of a mutation in the BUD4 gene. In addition, wild-type diploid strains lacking PHD1 undergo pseudohyphal growth when starved for nitrogen. The possible functions of PHD1 in pseudohyphal growth and the uses of the PHD screen to identify morphogenetic regulatory genes from heterologous organisms are discussed.