Genome sequence comparison of Aspergillus fumigatus strains isolated from patients with pulmonary aspergilloma and chronic necrotizing pulmonary aspergillosis
Genome sequence comparison of Aspergillus fumigatus strains isolated from patients with pulmonary aspergilloma and chronic necrotizing pulmonary aspergillosis
复制标题
肺曲霉瘤和慢性坏死性肺曲霉病患者烟曲霉菌株基因组序列比较
DOI：
10.1093/mmy/myv003
复制
发表时间：
2015
期刊：
影响因子：
2.9
通讯作者：
Takahashi H
中科院分区：
文献类型：
--
作者：
Takahashi-Nakaguchi A;Yasunori Muraosa Y;Hagiwara D;Sakai K,Toyotome T;Watanabe A;Kawamoto S;Kamei K;Gonoi T;Takahashi H
烟曲霉：最常与曲霉病相关的曲霉种类。在曲霉病的各种表现中，最常观察到的病例之一涉及。肺部烟曲菌感染是曲菌瘤（PA）。在这种感染中，人们发现真菌球由真菌菌丝、炎症细胞、纤维蛋白、粘液和组织碎片组成。慢性坏死性肺曲霉病（CNPA），也称为半侵袭性或侵袭性曲霉病，是局部侵袭性的，主要见于轻度免疫缺陷或慢性肺部疾病患者。在本研究中，我们利用新一代测序仪对日本PA和CNPA患者分离的17株菌株进行了全基因组测序（WGS）分析。通过将reads映射到A，共鉴定出99,088个snp。根据全基因组系统发育分析，全基因组序列分型结果与患者病理状况无相关性。在这里，我们进行了第一个多基因组WGS研究，重点关注theA。从PA和CNPA患者中分离出烟曲霉菌，并对菌株的遗传变异进行了综合表征。WGS方法将有助于更好地了解由a引起的曲霉病的分子机制。来自烟。
Aspergillus fumigatusis theAspergillusspecies most commonly associated with aspergillosis. Of the various presentations of aspergillosis, one of the most frequently observed in cases involvingA. fumigatuspulmonary infections is aspergilloma (PA). In such infections one finds a fungus ball composed of fungal hyphae, inflammatory cells, fibrin, mucus, and tissue debris. Chronic necrotizing pulmonary aspergillosis (CNPA), also known as semi-invasive or invasive aspergillosis, is locally invasive and predominantly seen in patients with mild immunodeficiency or with a chronic lung disease. In the present study, with the aid of a next-generation sequencer, we conducted whole genome sequence (WGS) analyses of 17 strains isolated from patients in Japan with PA and CNPA. A total of 99,088 SNPs were identified by mapping the reads toA. fumigatusgenome reference strain Af293, and according to genome-wide phylogenetic analysis, there were no correlations between the whole genome sequence typing results and pathologic conditions of patients. Here, we conducted the first multi-genome WGS study to focus on theA. fumigatusstrains isolated from patients with PA and CNPA, and comprehensively characterized genetic variations of strains. WGS approach will help in better understanding of molecular mechanisms of aspergillosis cases caused byA. fumigatus.