A Sortase A-Immobilized Mesoporous Hollow Carbon Sphere-Based Biosensor for Detection of Gram-Positive Bacteria
A Sortase A-Immobilized Mesoporous Hollow Carbon Sphere-Based Biosensor for Detection of Gram-Positive Bacteria
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DOI：
10.1007/s11664-018-6308-4
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发表时间：
2018-05
影响因子：
2.1
通讯作者：
Hongsu Wang;Ruiping Luo;Yang Chen;Qi Si;X. Niu
中科院分区：
文献类型：
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作者：
Hongsu Wang;Ruiping Luo;Yang Chen;Qi Si;X. Niu
报道了一种基于介孔碳材料的金黄色葡萄球菌（S. aureus）分选酶A（SrtA）传感器。为了制备生物传感器，我们首先合成了羧基功能化的介孔空心碳球，然后将其用作载体固定SrtA。基于SrtA的催化机制，建立了一种灵敏度高、成本低、快速检测金黄色葡萄球菌的方法。该传感器在0.125 × 102CFU mL− 1至2.5 × 102CFU mL−1的细菌浓度范围内显示出线性响应，检测限低至9.0 CFU mL−1。该方法已成功地用于全脂牛奶中金黄色葡萄球菌的定量检测，其结果与平板计数法的实验结果相似。该生物传感器也可用于检测其他分泌SrtA的革兰氏阳性菌。
A sensor based on mesoporous carbon materials immobilized with sortase A (SrtA) for determination ofStaphylococcus aureus(S. aureus) is reported. To prepare the biosensor, we first synthesized carboxyl-functionalized mesoporous hollow carbon spheres, then applied them as carriers for immobilization of SrtA. Based on the catalytic mechanism of SrtA, a highly sensitive, inexpensive, and rapid method was developed forS. aureusdetection. The sensor showed a linear response in the bacterial concentration range of 0.125 × 102colony-forming units (CFU) mL−1to 2.5 × 102CFU mL−1, with detection limit as low as 9.0 CFU mL−1. The method was successfully used for quantitative detection ofS. aureusin whole milk samples, giving results similar to experimental results obtained from the plate counting method. This biosensor could also be used to detect other Gram-positive bacteria that secrete SrtA.