Brain-derived neurotrophic factor promotes the differentiation of various hippocampal nonpyramidal neurons, including Cajal-Retzius cells, in organotypic slice cultures
Brain-derived neurotrophic factor promotes the differentiation of various hippocampal nonpyramidal neurons, including Cajal-Retzius cells, in organotypic slice cultures
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脑源性神经营养因子促进器官切片培养中各种海马非锥体神经元（包括 Cajal-Retzius 细胞）的分化
作者：
S. Marty;P. Carroll;A. Cellerino;E. Castrén;V. Staiger;H. Thoenen;D. Lindholm
脑源性神经营养因子（BDNF）在中枢神经系统中广泛表达，但对其功能知之甚少。本研究的目的是利用产后大鼠制备的器官切片培养物来研究 BDNF 对海马非锥体神经元分化的影响。 BDNF 的应用诱导了东方层非锥体神经元中微管相关蛋白 (MAP)-2 免疫染色的增加。生物胞素标记后的分析表明，BDNF 促进这些神经元树突的伸长。定向层中含有钙结合蛋白-D 和钙结合蛋白的非锥体细胞亚群对 BDNF 有反应，但对神经生长因子没有反应，如这些蛋白质免疫染色的神经元数量增加所示。 BDNF 还诱导东方层神经元的神经肽 Y 免疫染色增加。相反，BDNF 对小白蛋白免疫染色没有影响，尽管这些细胞表达 BDNF 受体 trkB。此外，BDNF 还增加了位于海马裂周围的 Cajal-Retzius 细胞中的钙结合蛋白免疫反应性。 Cajal-Retzius 神经元在体内退化的时间之后仍保留在切片中。然而，BDNF 并不是这些细胞生存所必需的，因为它们也持续存在于 BDNF 敲除小鼠的切片中。目前的结果表明 BDNF 对东方层非锥体细胞的形态及其钙结合蛋白水平产生影响。 BDNF 还调节 Cajal-Retzius 细胞的钙视网膜素含量，但并不是其生存所必需的。
Brain-derived neurotrophic factor (BDNF) is widely expressed in the central nervous system, where its function is poorly understood. The aim of this study was to investigate the effects of BDNF on the differentiation of hippocampal nonpyramidal neurons using organotypic slice cultures prepared from postnatal rats. The application of BDNF induced an increase in immunostaining for the microtubule-associated protein (MAP)-2 in non-pyramidal neurons of the stratum oriens. BDNF promotes the elongation of the dendrites of these neurons, as demonstrated by analysis after biocytin labeling. Calbindin-D- and calretinin-containing subgroups of nonpyramidal cells in the stratum oriens were responsive to BDNF but not to nerve growth factor, as shown by an increase in the number of neurons immunostained for these proteins. BDNF also induced an increase in neuropeptide Y immunostaining of stratum oriens neurons. In contrast, BDNF had no effect on parvalbumin immunostaining, despite the fact that these cells express the BDNF receptor trkB. In addition, BDNF increased calretinin immunoreactivity in Cajal-Retzius cells situated around the hippocampal fissure. The Cajal-Retzius neurons persisted in slices beyond the time at which they degenerate in vivo. However, BDNF is not required for the survival of these cells, because they also persisted in slices from BDNF knock-out mice. The present results indicate that BDNF exerts an effect on the morphology of stratum oriens nonpyramidal cells and their calcium-binding protein levels. BDNF also regulates the calretinin content of Cajal-Retzius cells but is not necessary for their survival.