Measurement of expansion factor and distortion for expansion microscopy using isolated renal glomeruli as landmarks
Measurement of expansion factor and distortion for expansion microscopy using isolated renal glomeruli as landmarks
复制标题
使用分离的肾小球作为标志测量膨胀显微镜的膨胀系数和畸变
DOI：
10.1002/jbio.202100001
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发表时间：
2021-04
影响因子：
2.8
通讯作者：
Huizhong Xu
中科院分区：
文献类型：
--
作者：
Chen Zhu;Aidong Wang;Lili Chen;Liangsheng Guo;Jiajia Ye;Qilin Chen;Qi Wang;Guojia Yao;Qin Xia;Tianyu Cai;Jiayun Guo;Zhenyu Yang;Zhenglong Sun;Yuwei Xu;Guoyuan Lu;Zexin Zhang;Jingyuan Cao;Ying Liu;Huizhong Xu
扩展显微镜已经实现了生物样品的超分辨率成像。膨胀因子和失真的精确测量通常需要在膨胀前后定位和成像样品中的相同感兴趣区域，这通常是耗时的。在这里，我们介绍了一种方便的方法，利用分离的猪肾小球作为地标，在扩张过程中重新定位。在热变性和蛋白酶K消化方案之后，肾小球表现出3.5至4的膨胀因子（仅比水凝胶膨胀少7%-16%）和1%至2%的相对变形。由于其100至300 μm的适当尺寸，肾小球在样品中的位置是肉眼可见的，而其详细形状仅需要明场显微镜。对于范围从3到10的扩展因子，肾小球附近的区域可以容易地重新识别，并且有时允许在没有荧光标记的明视野下量化扩展因子和失真。
Expansion microscopy has enabled super resolution imaging of biological samples. The accurate measurement of expansion factor and distortion typically requires locating and imaging the same region of interest in the sample before and after expansion, which is often time‐consuming to achieve. Here we introduce a convenient method for relocation by utilizing isolated porcine glomeruli as landmarks during expansion. Following heat denaturation and proteinase K digestion protocols, the glomeruli exhibit expansion factor of 3.5 to 4 (only 7%‐16% less expanded than the hydrogel), and 1% to 2% of relative distortion. Due to its appropriate size of 100 to 300 μm, the location of the glomerulus in the sample are visible to eyes, while its detailed shape only requires bright field microscopy. For expansion factors ranging from 3 to 10, the region in the vicinity of the glomerulus can be easily re‐identified, and sometimes allows quantification of expansion factor and distortion under bright field without fluorescent labels.
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光学成像。膨胀显微镜。
DOI：
10.1126/science.1260088
发表时间：
2015-01-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chen F;Tillberg PW;Boyden ES
通讯作者：
Boyden ES
影响因子：
56.9
作者：
Balzarotti, Francisco;Eilers, Yvan;Hell, Stefan W.
通讯作者：
Hell, Stefan W.
影响因子：
2.3
作者：
Pernal, Sebastian P.;Liyanaarachchi, Asiri;Jena, Bhanu P.
通讯作者：
Jena, Bhanu P.
影响因子：
16.6
作者：
Hale, Lorna J.;Howden, Sara E.;Little, Melissa H.
通讯作者：
Little, Melissa H.
影响因子：
46.9
作者：
Ku, Taeyun;Swaney, Justin;Park, Jeong-Yoon;Albanese, Alexandre;Murray, Evan;Cho, Jae Hun;Park, Young-Gyun;Mangena, Vamsi;Chen, Jiapei;Chung, Kwanghun
通讯作者：
Chung, Kwanghun