Immunocytochemical localization of the glucose transporter 2 (GLUT2) in the adult rat brain. II. Electron microscopic study
Immunocytochemical localization of the glucose transporter 2 (GLUT2) in the adult rat brain. II. Electron microscopic study
复制标题
DOI：
10.1016/j.jchemneu.2004.06.002
复制
发表时间：
2004-11-01
影响因子：
2.8
通讯作者：
Penicaud, L
中科院分区：
文献类型：
--
作者：
Arluison, M;Quignon, M;Penicaud, L
在大鼠脑中的先前免疫组织化学研究之后[Arluison，M.，Quignon，M.，阮，P.，Thorens，B.，Leloup，C.，佩尼科湖葡萄糖转运蛋白2（GLUT 2）在成年大鼠脑中的分布和解剖定位。I.免疫组织化学研究。神经化学杂志，我们分析了GLUT 2在前脑和后脑的代表性和/或关键区域的超微结构定位。与以前的结果一致，我们观察到少数少突胶质细胞和星形胶质细胞的细胞体离散标记为GLUT 2在大的有髓纤维束和大多数大脑区域检查，而反应性胶质细胞的过程是更多的，往往位于附近的神经末梢和/或树突或树突棘形成突触接触。其中只有一部分与未标记的神经细胞体和树突紧密结合。此外，神经细胞体显着免疫染色GLUT 2是稀缺的脑核检查，而标记的树突和树突棘是相对众多的，经常从事突触连接。与在不同脑区的许多神经细胞体的外周观察到GLUT 2免疫反应环一致（见以前的论文），我们在这里报告，一些神经元perikarya的背髓内核/嗅周皮质表现出一些补丁的免疫染色质膜下。然而，许多GLUT 2免疫反应神经末梢和/或星形胶质细胞的过程中，他们中的一些人偶尔被连接到神经细胞体和树突的存在下，也可以解释观察到的细胞周标记。这里报道的结果支持的想法，GLUT 2可能表达的一些大脑神经元可能参与葡萄糖传感，如前所述。然而，这也是可能的，这种转运蛋白参与调节神经递质的释放，也许，在释放葡萄糖的神经胶质细胞。(C)2004 Elsevier B. V.保留所有权利。
Following a former immunohistochemical study in the rat brain [Arluison, M., Quignon, M., Nguyen, P., Thorens, B., Leloup, C., Penicaud, L. Distribution and anatomical localization of the glucose transporter 2 (GLUT2) in the adult rat brain. I. Immunohistochemical study. J. Chem. Neuroanat., in press], we have analyzed the ultrastructural localization of GLUT2 in representative and/or critical areas of the forebrain and hindbrain. In agreement with previous results, we observe few oligodendrocyte and astrocyte cell bodies discretely labeled for GLUT2 in large myelinated fibre bundles and most brain areas examined, whereas the reactive glial processes are more numerous and often localized in the vicinity of nerve terminals and/or dendrites or dendritic spines forming synaptic contacts. Only some of them appear closely bound to unlabeled nerve cell bodies and dendrites. Furthermore, the nerve cell bodies prominently immunostained for GLUT2 are scarce in the brain nuclei examined, whereas the labeled dendrites and dendritic spines are relatively numerous and frequently engaged in synaptic junctions. In conformity with the observation of GLUT2-immunoreactive rings at the periphery of numerous nerve cell bodies in various brain areas (see previous paper), we report here that some neuronal perikarya of the dorsal endopiriform nucleus/perirhinal cortex exhibit some patches of immunostaining just below the plasma membrane. However, the presence of many GLUT2-immunoreactive nerve terminals and/or astrocyte processes, some of them being occasionally attached to nerve cell bodies and dendrites, could also explain the pericellular labeling observed. The results here reported support the idea that GLUT2 may be expressed by some cerebral neurones possibly involved in glucose sensing, as previously discussed. However, it is also possible that this transporter participate in the regulation of neurotransmitter release and, perhaps, in the release of glucose by glial cells. (C) 2004 Elsevier B.V. All rights reserved.