Phytochemical analysis and geographic assessment of flavonoids, coumarins and sesquiterpenes in Artemisia annua L. based on HPLC-DAD quantification and LC-ESI-QTOF-MS/MS confirmation
Phytochemical analysis and geographic assessment of flavonoids, coumarins and sesquiterpenes in Artemisia annua L. based on HPLC-DAD quantification and LC-ESI-QTOF-MS/MS confirmation
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基于 HPLC-DAD 定量和 LC-ESI-QTOF-MS/MS 确认的青蒿中黄酮类、香豆素类和倍半萜类的植物化学分析和地理评估
DOI：
10.1016/j.foodchem.2019.126070
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发表时间：
2020-05-15
期刊：
影响因子：
8.8
通讯作者：
Qiu, Feng
中科院分区：
文献类型：
--
作者：
Fu, Chunqing;Yu, Ping;Qiu, Feng
为评价青蒿药材的质量，建立了准确的高效液相色谱分离分析方法，并用于5种黄酮类成分、2种香豆素类化合物和4种倍半萜类化合物的同时定量。采用LC-ESI-QTOF-MS/MS确认方法，避免了假阳性结果。主成分分析和系统聚类分析结果表明，它们的内容具有明显的区域特征。青蒿素(8.24+/-2.92 mg/g)和异鼠李素(0.28+/-0.25 mg/g)含量较高的样品主要分布在长江以南地区，东五味素(0.46+/-0.22 mg/g)、东七内酯(1.05+/-0.17 mg/g)、大黄花酚D(0.64+/-0.14 mg/g)、酪蛋白(1.07+/-0.23 mg/g)、青蒿素B(0.69+/-0.18 mg/g)和青蒿酸(3.02+/-1.00 mg/g)主要分布在中国东部和北部。不同地区青蒿中各成分的含量差异表明其临床应用的健康促进作用存在潜在差异。
To evaluate the quality of Artemisia annua L., an accurate HPLC-DAD method has been developed, validated and applied to the simultaneous quantification of five flavonoids, two coumarins and four sesquiterpenes. An LC-ESI-QTOF-MS/MS confirmation method has been utilized to avoid false-positive results. Principal component analysis and hierarchical cluster analysis results indicated that their contents had obvious regional characteristics. Samples with high contents of artemisinin (8.24 +/- 2.92 mg/g) and isorhamnetin (0.28 +/- 0.25 mg/g) are mainly distributed south of the Yangtze River, and samples with high contents of scopolin (0.46 +/- 0.22 mg/g), scopoletin (1.05 +/- 0.17 mg/g), chrysosplenol D (0.64 +/- 0.14 mg/g), casticin (1.07 +/- 0.23 mg/g), arteannuin B (0.69 +/- 0.18 mg/g) and artemisinic acid (3.02 +/- 1.00 mg/g) are mainly distributed in eastern and northern China. Geographic content differences of the components in A. annua indicate the potential differences in the health-promoting effects of its clinical application.