On the purification and mechanism of action of 5-aminoimidazole-4-carboxamide-ribonucleotide transformylase from chicken liver.
On the purification and mechanism of action of 5-aminoimidazole-4-carboxamide-ribonucleotide transformylase from chicken liver.
复制标题
鸡肝中5-氨基咪唑-4-甲酰胺-核糖核苷酸转化酶的纯化及作用机制研究
DOI：
10.1021/bi00505a017
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Benkovic,SJ
中科院分区：
文献类型：
--
作者：
Mueller,WT;Benkovic,SJ
5-氨基-4-咪唑甲酰胺-核糖核苷酸转化酶（EC 2.1. 2.3，AICAR TFase），参与嘌呤生物合成的两种需要还原叶酸的转化酶之一，催化AICAR与10-甲酰-H4叶酸的缩合以产生FAICAR和H4叶酸。Flaks等人已经从鸡肝中部分纯化了该酶。（1957）、Baggott和Krumdieck（1979 a）（分别为41- 158倍）和盖革和Guglielini（1975）（分别为63倍）。肌苷酶（EC 3.5. 4.10，IMP环化水解酶）在嘌呤生物合成途径中紧随AICAR TF酶，将FAICAR环化为IMP。在所有上述纯化中，据报道该酶与转化酶共纯化（Baggott和Krumdieck，1979 b），这引起了这两种活性紧密相关或在同一蛋白上的推测。
5-Amino-4-imidazolecarboxamide-ribonucleotide transformylase (EC 2.1. 2.3, AICAR TFase), 1 one of two reduced folate-requiring transformylases involved in purinebiosynthesis, catalyzes the formylation of AICAR with 10-formyl-H4folate to produce FAICAR and H4folate. The enzyme has been partially purified from chicken liver by Flaks et al.(1957), by Baggott & Krumdieck (1979a)(41-to 158-fold, respec-tively), and 63-foldfrom Ehrlich Ascites tumor cells by Geiger & Guglielini (1975). Inosinicase (EC 3.5. 4.10, IMP cyclohydrolase) immediately follows AICAR TFase in the purine biosynethic pathway, cyclizing FAICAR to IMP. In all of the above purifications this enzyme was reported to copurify with the transformylase (Baggott & Krumdieck, 1979b), which gave rise to speculation that the two activities were either tightly associated or were on the same protein.