Double immunolabeling of central nervous system atypical teratold/rhabdoid tumors
Double immunolabeling of central nervous system atypical teratold/rhabdoid tumors
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DOI：
10.1038/modpathol.3800099
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发表时间：
2004-06-01
期刊：
影响因子：
7.5
通讯作者：
Rorke, LB
中科院分区：
文献类型：
--
作者：
Bouffard, JP;Sandberg, GD;Rorke, LB
中枢神经系统非典型畸胎瘤样/横纹肌样瘤（ATRT）是一种高度恶性肿瘤，具有异质性免疫组织化学特征，并且与中枢神经系统原始神经外胚层肿瘤（PNET）具有一定的形态相似性。尽管有几项研究调查了 PNET 中的双重免疫标记，但我们知道还没有关于 ATRT 双重标记的研究。从费城儿童医院的手术和咨询材料中选择了总共 10 个 ATRT，并使用间接免疫荧光对多种抗原进行染色，以检测单标记和双标记。大多数肿瘤细胞仅显示单一标记；罕见细胞显示如下双重标记：70％的肿瘤共表达（VIM）和神经胶质纤维酸性蛋白（GFAP），30％平滑肌肌动蛋白和GFAP，20％上皮膜抗原（EMA）和VIM，20％EMA/GFAP和20％EMA/SMA。鉴于当前关于 CNS PNET 和 ATRT 组织发生的争论，并参考横纹肌样肿瘤作为实体或表型的分类，讨论了这些结果。
The central nervous system atypical teratoid/rhabdoid tumor (ATRT) is a highly malignant tumor with a heterogeneous immumohistochemical profile and with some morphologic similarity to central nervous system primitive neuroectodermal tumors (PNET). Although several studies have investigated double immunolabeling in PNET, we are aware of no studies of double labeling of ATRT. A total of 10 ATRT from surgical and consultation materials at the Children's Hospital of Philadelphia were selected and stained for a variety of antigens using indirect immunofluorescence to detect single and double labeling. Most tumor cells showed only single labeling; rare cells showed double labeling as follows: 70% of tumors coexpressed (VIM) and glial flibrillary acidic protein (GFAP), 30% smooth muscle actin and GFAP, 20% epithellial membrane antigen (EMA) and VIM, 20% EMA/GFAP, and 20% EMA/SMA. These results are discussed in view of current debates over the histogenesis of CNS PNET and ATRT, and in reference to the classification of rhabdoid tumors as an entity or phenotype.