Cell Death of Rice Roots under Salt Stress May Be Mediated by Cyanide-Resistant Respiration
Cell Death of Rice Roots under Salt Stress May Be Mediated by Cyanide-Resistant Respiration
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盐胁迫下水稻根部细胞死亡可能是由抗氰呼吸介导的
DOI：
10.5560/znc.2013.68c0039
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发表时间：
2013-01-01
影响因子：
2
通讯作者：
Ding, Yanping
中科院分区：
文献类型：
--
作者：
Feng, Hanqing;Hou, Xiuli;Ding, Yanping
高浓度氯化钠(200 mM或300 mM)处理可诱导水稻细胞死亡。根，以及外源过氧化氢(过氧化氢)的应用。此外，过氧化氢清除剂二甲基硫脲(DMTU)可部分缓解200 mM氯化钠诱导的根细胞死亡。这些观察结果表明，水稻根在高盐胁迫下的细胞死亡与体内过氧化氢的积累有关。盐胁迫在一定程度上提高了水稻根系的抗氰呼吸水平，提高了交替氧化酶基因AOX1a和AOX1b的转录水平。在高盐胁迫(200 MM NaC L)下，用1 mM水杨基异羟肟酸(SHAM)处理的水稻根的细胞死亡和过氧化氢产生水平高于200 mM NaC l胁迫或单独的SHAM处理。这些结果表明，抗氰化物呼吸可能在高盐胁迫下介导根细胞死亡中发挥作用。此外，抗氰化物呼吸的这种功能可能与其减少过氧化氢产生的能力有关。
Treatment with solutions containing high concentrations of NaCl (200 or 300 mM) induced cell death in rice (Oryza sativa L.) roots, as well as the application of exogenous hydrogen peroxide (H2O2). Moreover, the pretreatment with dimethylthiourea (DMTU), a scavenger of H2O2, partially alleviated the root cell death induced by 200 mM NaCl. These observations suggest that the cell death of rice roots under high salt stress is linked to H2O2 accumulation in vivo. NaCl stress increased the level of cyanide-resistant respiration to some extent and enhanced the transcript levels of the alternative oxidase (AOX) genes AOX1a and AOX1b in rice roots. High-salt-stressed (200 mM NaCl) rice roots pretreated with 1 mM salicylhydroxamic acid (SHAM), a specific inhibitor of alternative oxidase, exhibited higher levels of cell death and H2O2 production than roots subjected to either 200 mM NaCl stress or SHAM treatment alone. These results suggest that cyanide-resistant respiration could play a role in mediating root cell death under high salt stress. Furthermore, this function of cyanide-resistant respiration could relate to its ability to reduce the generation of H2O2.