Gene expression profiles, of BRCA1-linked, BRCA2-linked, and sporadic ovarian cancers
Gene expression profiles, of BRCA1-linked, BRCA2-linked, and sporadic ovarian cancers
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DOI：
10.1093/jnci/94.13.990
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发表时间：
2002-07-03
影响因子：
10.3
通讯作者：
Liu, ET
中科院分区：
文献类型：
--
作者：
Jazaeri, AA;Yee, CJ;Liu, ET
背景资料：BRCA 1和BRCA 2的生殖系突变导致5%-10%的上皮性卵巢癌，但受这些突变影响的分子途径尚不清楚。我们使用互补DNA（cDNA）微阵列来比较与BRCA 1或BRCA 2突变相关的卵巢癌的基因表达模式与散发性上皮性卵巢癌的基因表达模式，并确定遗传性和散发性肿瘤的共同模式。研究方法：研究了61例病理证实的上皮性卵巢腺癌患者的肿瘤样本，这些患者具有匹配的临床病理特征，包括18例BRCA 1创始人突变，16例BRCA 2创始人突变，27例无创始人突变（称为散发性癌症）。cDNA微阵列包含7651个序列验证特征。基因表达数据采用改良双侧F检验进行分析，P
Background: Germline mutations in BRCA1 and BRCA2 are responsible for 5%-10% of epithelial ovarian cancers, but the molecular pathways affected by these mutations are unknown. We used complementary DNA (cDNA) microarrays to compare gene expression patterns in ovarian cancers associated with BRCA1 or BRCA2 mutations with gene expression patterns in sporadic epithelial ovarian cancers and to identify patterns common to both hereditary and sporadic tumors. Methods: Tumor samples from 61 patients with pathologically confirmed epithelial ovarian adenocarcinoma with matched clinicopathologic features were studied, including 18 with BRCA1 founder mutations, 16 with BRCA2 founder mutations, and 27 without either founder mutation (termed sporadic cancers). The cDNA microarrays contained 7651 sequence-verified features. Gene expression data were analyzed with a modified two-sided F test, with P