Genetic polymorphism of interleukin-17A and-17F genes in gastric carcinogenesis
Genetic polymorphism of interleukin-17A and-17F genes in gastric carcinogenesis
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DOI：
10.1016/j.humimm.2009.04.030
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发表时间：
2009-07-01
期刊：
影响因子：
2.7
通讯作者：
Arisawa, Tomiyasu
中科院分区：
文献类型：
--
作者：
Shibata, Tomoyuki;Tahara, Tomomitsu;Arisawa, Tomiyasu
白介素17A(IL-17A)和白介素17F(IL-17F)通过诱导促炎细胞因子和中性粒细胞动员细胞因子的释放在组织炎症中发挥作用。我们研究了IL-17A(rs2275913，G-197A)和-17F(rs763780,7488 T/C)基因多态性与胃癌的关系。这项研究是在811名受试者中进行的(524名非胃癌患者和287名胃癌患者)。采用多重聚合酶链式反应-单链构象多态性方法检测基因多态性。总体而言，IL-17A/-197A等位基因数目与胃癌的发生密切相关(OR=1.42；95%CI：1.09~1.85；p=0.010)。胃癌组IL-17A/-197A/A纯合子频率也显著高于非癌组(OR，3.02；95%CI，1.86~4.91：P<；0.0001)。IL-17A/G-197A基因多态性与肠型癌的相关性高于弥漫型癌。此外，IL-17A/-197A等位基因携带者发生胃粘膜萎缩的风险增加(OR为1.68；95%CI为1.06~2.65；p=0.026)，炎症积分与-197A等位基因数目呈正相关(p=0.022)。提示IL-17A基因G-197A多态性与胃癌尤其是肠型癌的发生发展密切相关。(C)2009年，爱思唯尔公司代表美国组织相容性和免疫遗传学会出版。
Interleukin-17A (IL-17A) and IL-17F play a role in tissue inflammation by inducing release of proinflammatory and neutrophil-mobilizing cytokines. We investigated the associations between gastric cancer and polymorphisms of IL-17A (rs2275913, G-197A) and -17F (rs763780, 7488 T/C) genes. The study was performed in 811 Subjects (524 without gastric cancer and 287 with gastric cancer). We used the multiplex PCR-SSCP method to detect gene polymorphisms. Overall, the number of IL-17A/-197A allele was significantly correlated to the development of gastric cancer (OR, 1.42; 95% Cl, 1.09 -1.85; p = 0.010). The frequency of IL-17A/-197 A/A homozygote was also significantly higher in gastric cancer group than in non-cancer group (OR, 3.02; 95% Cl, 1.86-4.91: p < 0.0001). The IL-17A/G-197A polymorphism was more closely correlated to intestinal-type cancer than diffuse-type cancer. In addition, IL-17A/-197A allele carriers had an increased risk of the development of gastric mucosal atrophy (OR, 1.68; 95% CI, 1.06-2.65; p = 0.026), and a positive relationship between the inflammation score and the number of -197A allele was observed (p = 0.022). We concluded that G-197A polymorphism of IL-17A gene was significantly associated with the development of gastric cancer, especially intestinal-type cancer. (C) 2009 Published by Elsevier Inc. on behalf of American Society for Histocompatibility and Immunogenetics.