Integrated Analysis of Hub Genes and miRNAs in Dilated Cardiomyopathy.
Integrated Analysis of Hub Genes and miRNAs in Dilated Cardiomyopathy.
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扩张型心肌病 Hub 基因和 miRNA 的综合分析
DOI：
10.1155/2020/8925420
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发表时间：
2020
影响因子：
--
通讯作者：
Sun X
中科院分区：
文献类型：
--
作者：
Huang K;Wen S;Huang J;Wang F;Pang L;Wang Y;Sun X
本研究的目的是通过miRNA-mRNA相互作用网络识别扩张型心肌病(DCM)疾病中的Hub基因和miRNAs。使用从GEO数据库(GSE112556和GSE3585)下载的DCM患者的数据筛选差异表达的miRNAs(Demis)和mRNAs(Dem)。用基因本体论(GO)途径分析和转录因子富集法筛选DEMI，用miRDB、miRTarBase和TargetScan筛选目的mRNAs。用Cytoscape软件可视化miRNAs和mRNAs之间的网络并计算HUB基因。GO和京都基因和基因组百科全书(KEGG)通路分析用于分析调控网络中的mRNAs。共筛选出9个DEMs和281个DEM，构建了由7个miRNAs和51个mRNAs组成的miRNA-mRNA网络。前10个节点miR-144-3p、miR-363-3p、miR-9-3p、miR-21-3p、miR-144-5p、miR-338-3p、ID4(脱氧核糖核酸结合/分化抑制因子4)、miR-770-5p、PIK3R1(P85α调节亚基)和FN1(纤维连接蛋白1)被确定为重要的调控基因。本研究发现了几个与DCM发病有关的重要HUB基因和miRNAs，其中miR-144-3p/FN1和miR-9-3p/FN1通路可能在心肌纤维化中发挥重要作用，有助于确定DCM的病因，并提供潜在的治疗靶点。
The aim of this study is to identify hub genes and miRNAs by the miRNA-mRNA interaction network in dilated cardiomyopathy (DCM) disease. The differentially expressed miRNAs (DEMis) and mRNAs (DEMs) were selected using data of DCM patients downloaded from the GEO database (GSE112556 and GSE3585). Gene Ontology (GO) pathway analysis and transcription factor enrichment analysis were used for selecting DEMis, and the target mRNAs of DEMis were filtered by using miRDB, miRTarBase, and TargetScan. Cytoscape software was used to visualize the network between miRNAs and mRNAs and calculate the hub genes. GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to analyze the mRNAs in the regulatory network. A total of 9 DEMis and 281 DEMs were selected, from which we reconstructed the miRNA-mRNA network consisting of 7 miRNAs and 51 mRNAs. The top 10 nodes, miR-144-3p, miR-363-3p, miR-9-3p, miR-21-3p, miR-144-5p, miR-338-3p, ID4 (inhibitor of DNA binding/differentiation 4), miR-770-5p, PIK3R1 (p85α regulatory subunit of phosphoinositide 3-kinase (PI3K)), and FN1 (fibronectin 1), were identified as important regulators. The study uncovered several important hub genes and miRNAs involved in the pathogenesis of DCM, among which, the miR-144-3p/FN1 and miR-9-3p/FN1 pathways may play an important role in myocardial fibrosis, which can help identify the etiology of DCM, and provide potential therapeutic targets.
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影响因子：
24
作者：
Barth, Andreas S.;Kuner, Ruprecht;Sueltmann, Holger
通讯作者：
Sueltmann, Holger
影响因子：
8.3
作者：
Verjans, Robin;Peters, Tim;Schroen, Blanche
通讯作者：
Schroen, Blanche
影响因子：
6.2
作者：
Kang, Sean;Verma, Subodh;Fedak, Paul W. M.
通讯作者：
Fedak, Paul W. M.
DOI：
10.1038/nrm3313
发表时间：
2012-03-22
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
39.3
作者：
Elliott, Perry;Andersson, Bert;Keren, Andre
通讯作者：
Keren, Andre