Enhancement of sperm motility and viability by turmeric by-product dietary supplementation in roosters
Enhancement of sperm motility and viability by turmeric by-product dietary supplementation in roosters
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DOI：
10.1016/j.anireprosci.2017.08.021
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发表时间：
2017-10-01
影响因子：
2.2
通讯作者：
Kawahara, Manabu
中科院分区：
文献类型：
--
作者：
Yan, Wenjing;Kanno, Chihiro;Kawahara, Manabu
提高精子活力和存活力是提高家禽业效率的主要目标。在这项研究中，补充膳食姜黄副产品（TBP）的影响，从商业姜黄生产精子活力，活力和抗氧化状态进行了研究。将成熟的罗得岛红公鸡分为两组：对照组（C组），不施用TBP;试验组（T组），在温控饲养设施中饲喂添加0.8 g TBP/天的基础饮食（试验1），在热应激下饲喂添加1.6 g/天的基础饮食（试验2），持续4周。在实验1中，TBP饮食补充增加精子活力变量直线速度，曲线速度，和线性的基础上，计算机辅助精液分析，2周后TBP补充。在实验2中，使用流式细胞术，精子活力在3和4周后TBP补充T组大于C组，这种增加与活性氧（ROS）的生产减少在2和4周。这两个实验的结果清楚地表明，饮食中补充TBP提高精子活力在控制温度的条件下，以及精子的活力，并减少ROS的产生时，热应激盛行。考虑到其在一系列环境中的潜在应用，TBP可以作为一种经济有效的抗氧化剂来提高公鸡的繁殖力。
Improving sperm motility and viability are major goals to improve efficiency in the poultry industry. In this study, the effects of supplemental dietary turmeric by-product (TBP) from commercial turmeric production on sperm motility, viability, and antioxidative status were examined in domestic fowl. Mature Rhode Island Red roosters were divided into two groups controls (group C) without TBP administration and test subjects (group T) fed a basal diet supplemented with 0.8 g of TBP/day in a temperature-controlled rearing facility (Experiment 1) and 1.6 g/day under heat stress (Experiment 2) for 4 weeks. In Experiment 1, TBP dietary supplementation increased the sperm motility variables straight-line velocity, curvilinear velocity, and linearity based on a computer-assisted semen analysis, 2 weeks following TBP supplementation. In Experiment 2, using flow cytometry, sperm viability at 3 and 4 weeks following TBP supplementation was greater in Group T than C, and this increase was consistent with a reduction in reactive oxygen species (ROS) production at 2 and 4 weeks. The results of both experiments clearly demonstrate that dietary supplementation with TBP enhanced sperm motility in the controlled-temperature conditions as well as sperm viability, and reduced ROS generation when heat stress prevailed. Considering its potential application in a range of environments, TBP may serve as an economical and potent antioxidant to improve rooster fertility.