DNA duplexes flanked by hybrid duplexes: the solution structure of chimeric junctions in [r(cgcg)d(TATACGCG)]2.
DNA duplexes flanked by hybrid duplexes: the solution structure of chimeric junctions in [r(cgcg)d(TATACGCG)]2.
复制标题
两侧为杂合双链体的 DNA 双链体：[r(cgcg)d(TATACGCG)]2 中嵌合连接的溶液结构。
DOI：
10.1021/bi00007a033
复制
发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Reid,BR
中科院分区：
文献类型：
--
作者：
Zhu,L;Salazar,M;Reid,BR
Revised Manuscript Received December 2, 1994® abstract; Hybrid duplexes and chimeric duplexes containinghybrid segments linked to pure DNA (or pure RNA) segments are involved in transcription and replication, as well as reverse transcription. A complete understanding of the mechanism of these processes requires detailed information on such duplexes and the junctions betweenduplexes of differing structure. Using two-dimensional NMR, restrained molecular dynamics and mechanics, and back-calculation refinement against the nuclear Overhauser effect spectra at various mixing times, we have determined the solution structure of the chimeric duplex [r (cgcg) d-(TATACGCGjja containing a pure DNA segment in the center of a hybrid duplex. The solution structure differs from the previously determined X-ray structure of the analogous duplex [r (gcg) d (TATACGC)] 2, which was found tobe A-form throughout [Wang, A. H.-J., et al.(1982) Nature 299, 601—604], The basic features of the solution structure are (a) the RNA residues are all A-form with C3'-endo sugar conformations,(b) the central DNA segment is B-form,(c) the transition from A-form RNA sugar conformations to B-form DNA sugar conformations involves only the dT5 base step, and (d) although the sugar conformations of the DNA residues A6—G12 are closer to B-form, the basic helical properties of the peripheral RNA-DNA hybrid segments are closer to typical A-form than to B-form.
