Pit formation on the basal plane of ice in antifreeze protein type III solution for different growth mechanism of ice
Pit formation on the basal plane of ice in antifreeze protein type III solution for different growth mechanism of ice
复制标题
不同冰生长机制的III型抗冻蛋白溶液中冰基面凹坑的形成
DOI：
10.1021/acs.cgd.5b01596
复制
发表时间：
2016
影响因子：
3.8
通讯作者：
Kunio Funakoshi
中科院分区：
文献类型：
--
作者：
Takaaki Inada;Toshie Koyama;Kunio Funakoshi
当具有十分之几mm 2 in面积的基面的冰的单晶在中等活性的抗冻蛋白（AFP）溶液中生长时，在基面上形成由六个锥体平面组成的许多凹坑。这种坑的形成表明AFP和基面之间存在一些相互作用。在这项研究中，我们观察到坑的形成在5毫克/毫升的鱼AFP（III型）溶液中生长的冰的基面上，并检查三个生长机制（正常，螺旋形，和二维（2D）成核）的基面通过测量的增长率和过冷度之间的关系。我们还测量了冰中的凹坑数密度，发现在分子粗糙表面上正常生长模式的凹坑数密度低于在相对光滑表面上螺旋生长模式的凹坑数密度，而在2D成核生长模式期间没有观察到凹坑形成。在此基础上，提出了螺旋生长模式下凹坑形成的模型。在这个模型中，如果只可逆吸附的AFP分子被认为是发生在光滑的表面上，那么坑的形成可以解释，而不假设不可逆的吸附在光滑的表面上。
When a single crystal of ice that has the basal plane several tenths of mm2in area grows in a moderately active antifreeze protein (AFP) solution, numerous pits consisting of six pyramidal planes are formed on the basal plane. This pit formation suggests some interactions between the AFPs and the basal plane. In this study, we observed pit formation on the basal plane of ice growing in a 5 mg/mL fish AFP (type III) solution and examined three growth mechanisms (normal, spiral, and two-dimensional (2D) nucleation) of the basal plane by measuring the relationship between the growth rate and the degree of supercooling. We also measured the number density of pits in ice and found that the number density of pits for normal growth mode on molecularly rough surfaces was lower than that for spiral growth mode on relatively smooth surfaces, whereas pit formation was not observed during 2D nucleation growth mode. On the basis of these results, we proposed a model of pit formation during spiral growth mode. In this model, if only reversible adsorption of the AFP molecules on the smooth surfaces is considered to occur, then pit formation can be explained without assuming irreversible adsorption on the smooth surfaces.