A procedure for assessing the salt tolerance of lucerne (Medicago sativa L.) cultivar seedlings by combining agronomic and physiological indicators
A procedure for assessing the salt tolerance of lucerne (Medicago sativa L.) cultivar seedlings by combining agronomic and physiological indicators
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DOI：
10.1080/00288230909510525
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发表时间：
2009-12
影响因子：
1.8
通讯作者：
Bao Aike;Guo Zheng-gang;Zhang Hong-fei;Wang Suo-min
中科院分区：
文献类型：
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作者：
Bao Aike;Guo Zheng-gang;Zhang Hong-fei;Wang Suo-min
摘要我国西部干旱半干旱地区灌溉引起的盐碱化对卢塞恩草地的牧草产量构成威胁。为筛选耐盐性较好的卢塞恩品种，以5个卢塞恩品种为材料，通过苗期地上部干重、地上部KVNa+比值、叶片丙二醛（MDA）含量和膜相对透性（RMP）等指标，对苜蓿品种的相对耐盐性进行了评价。NaCl处理降低了苜蓿地上部干重和地上部K+/Na+比值，提高了叶片MDA含量和RMP，但不同卢塞恩品种降低或提高的程度不同。在200 mM NaCl处理5天后，‘漫步者’的地上部干重显著高于其他品种（仅下降17.2%至0.17 g植株-1），并且也保持了较好的离子状态，具有较高的K+含量，较低的Na+含量，因此具有较高的相对地上部K+/Na+比。‘大叶’叶片中MDA含量和RMP均未显著增加，表现出较好的健康性。综合各指标的相对值，5个品种的相对耐盐性依次为：漫步者、大叶、三丰、甘农3号、陇东。本研究表明，‘漫步者’和‘大叶’可能适合在我国西部干旱半干旱地区的水浇地建植牧草。
Abstract Salinisation caused by irrigation is threatening the forage yield of lucerne pasture in the arid and semi‐arid areas of west China. To select lucerne cultivars with good salt tolerance, the relative salt tolerance of five lucerne cultivars was assessed based upon shoot dry weight, shoot KV Na+ ratio, leaf malondialdehyde (MDA) content and relative membrane permeability (RMP) at the seedling stage. NaCl reduced the shoot dry weight and shoot K+/Na+ ratio, and enhanced the leaf MDA content and RMP in all cultivars, but the degree of either reduction or enhancement differed between these lucerne cultivars. Under 200 mM NaCl for 5 days, ‘Rambler’ had significantly greater shoot dry weight (only dropped 17.2% to 0.17 g plant‐1) than other cultivars, and also maintained a better ion status, with higher K+ content, lower Na+ content, and thus a higher relative shoot K+/Na+ ratio. ‘Daye’ was healthier because neither the MDA content nor the RMP was significantly increased in its leaves. Through combining the relative values of these indicators, the relative salt tolerance of the five cultivars decreased in the order: ‘Rambler’, ‘Daye’, ‘Sandili’, ‘Gannong No.3’, and ‘Longdong’. This study indicated that ‘Rambler’ and ‘Daye’ might be suitable for pasture establishment on irrigated land in arid and semi‐arid areas of west China.