Functional analysis of structural motifs in dicistroviruses
Functional analysis of structural motifs in dicistroviruses
复制标题
DOI：
10.1016/j.virusres.2008.06.006
复制
发表时间：
2009-02-01
期刊：
影响因子：
5
通讯作者：
Uchiumi, Toshio
中科院分区：
文献类型：
--
作者：
Nakashima, Nobuhiko;Uchiumi, Toshio
双链病毒科由正链RNA病毒组成，其具有单部分基因组。这些病毒携带基因组连接的病毒蛋白（VPg）和poly（A）尾巴。5'非翻译区（UTR）约为500个核苷酸，含有一个内部核糖体进入位点（IRES）。这些特征类似于脊椎动物小核糖核酸病毒，但双顺反子病毒具有其他独特的特征。小核糖核酸病毒具有单个大的开放阅读框（ORF），其编码T末端的衣壳蛋白和T末端的复制酶。相反，双顺反子病毒有两个不重叠的ORB。5 '-近端ORF编码复制酶，T-近端ORF编码衣壳蛋白。通常，在基因组的3'部分具有衣壳蛋白基因的正链病毒产生用于合成衣壳蛋白的亚基因组RNA，因为病毒复制周期需要大量的衣壳蛋白。在双顺反子病毒中，衣壳蛋白的翻译由另外的IRES控制。该[RES]位于复制酶和衣壳编码区之间的基因间区（IGR）中，并且介导衣壳蛋白的翻译起始。IGR-IRES具有包含三个假结的多茎环结构。我们描述了双顺反子病毒的特征，包括RNA元件和病毒蛋白。(c)2008 Elsevier B. V.保留所有权利。
The family Dicistroviridae is composed of positive-stranded RNA viruses which have monopartite genomes. These viruses carry genome-linked virus proteins (VPg)and poly (A) tails. The 5' untranslated region(UTR) is approximately 500 nucleotides and contains an internal ribosome entry site (IRES). These features resemble those of vertebrate picornaviruses, but dicistroviruses have other distinct characteristics. Picornaviruses have a single large open reading frame (ORF) encoding the capsid proteins at the T-end and the replicases; at the T-end. In contrast, dicistroviruses have two nonoverlapping ORB. The 5'-proximal ORF encodes the replicases and the T-proximal ORF encodes the capsid proteins. Usually, positive-stranded viruses which have capsid protein genes in the 3' part of the genome produce subgenomic RNA for synthesis of the capsid proteins, because abundant quantities of the capsid proteins are required for the viral replication cycle. In dicistroviruses, translation of the capsid proteins is controlled by an additional IRES. This [RES is located in the intergenic region (IGR) between the replicase and capsid coding regions, and mediates the initiation of translation for the capsid proteins. The IGR-IRES has a multiple stem-loop structure containing three pseudoknots. We describe the characteristics of dicistroviruses, including the RNA elements and viral proteins. (c) 2008 Elsevier B.V. All rights reserved.