Early signal transduction induced by Candida albicans in macrophages through shedding of a glycolipid
Early signal transduction induced by Candida albicans in macrophages through shedding of a glycolipid
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DOI：
10.1086/515361
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发表时间：
1998-09-01
影响因子：
6.4
通讯作者：
Poulain, D
中科院分区：
文献类型：
--
作者：
Jouault, T;Fradin, C;Poulain, D
细胞壁 β-1,2-低聚甘露糖苷参与白色念珠菌与巨噬细胞的结合并刺激其产生细胞因子。研究了巨噬细胞 J774 细胞系与白色念珠菌初始相互作用期间发生的信号事件的性质，以及酵母释放的含有 β-1,2-低聚甘露糖苷的分子的性质。共培养导致产生除草霉素 A 敏感的肿瘤坏死因子-cy，免疫荧光和蛋白质印迹证实了酪氨酸磷酸化，并揭示了 90 至 120 kDa 磷蛋白的积累。 β-1,2-低聚甘露糖苷特异性抗体表明，这些表位在早期从酵母脱落到巨噬细胞膜，与先前描述为白色念珠菌磷脂甘露聚糖的糖脂相关。单独用纯化的磷脂甘露聚糖孵育巨噬细胞导致与活酵母观察到的信号转导途径相同，所有这些结果表明白色念珠菌磷脂甘露聚糖脱落参与C，白色念珠菌-巨噬细胞通过 β-1,2-低聚甘露糖苷相互作用。
Cell wall beta-1,2-oligomannosides are involved in Candida albicans binding to macrophages and in their stimulation to produce cytokines, The nature of signaling events occurring during initial interaction of macrophage J774 cell line and C, albicans, together with the nature of molecules containing beta-1,2-oligomannosides released by the yeasts, was examined. Cocultivation led to a herbimycin A-sensitive production of tumor necrosis factor-cy, Immunofluorescence and Western blotting confirmed tyrosine phosphorylation and revealed an accumulation of 90- to 120-kDa phosphoproteins. Antibodies specific for beta-1,2-oligomannosides showed that these epitopes were shed at an early stage from the yeasts to the macrophage membrane, in association with a glycolipid previously described as C. albicans phospholipomannan, Incubation of macrophages with purified phospholipomannan alone led to a signal transduction pathway identical to that observed with living yeasts, AU of these results demonstrate that C. albicans phospholipomannan shedding is involved in C, albicans-macrophage interaction through beta-1,2-oligomannosides.