Deletion of BK channels decreased skeletal and cardiac muscle function but increased smooth muscle contraction in rats
Deletion of BK channels decreased skeletal and cardiac muscle function but increased smooth muscle contraction in rats
复制标题
删除 BK 通道会降低大鼠的骨骼和心肌功能，但会增加平滑肌收缩
DOI：
10.1016/j.bbrc.2021.07.027
复制
发表时间：
2021-07-13
影响因子：
3.1
通讯作者：
Liu, Huirong
中科院分区：
文献类型：
--
作者：
He, Chunyu;Li, Xiaoyue;Liu, Huirong
Large conductance calcium-activated potassium channel (BK channel) is widely expressed in skeletal muscle, myocardium, smooth muscle and other muscle tissues. Mutation, abnormal expression and altered activity of BK channel are linked to muscle-related diseases such as dyskinesia, epilepsy and erectile dysfunction. In order to compare the effects of BK channel on different muscle tissues, we constructed BK channel gene knockout rats (BK-/- rats). HE staining, open field and grip strength tests, ultrasound, blood pressure measurement and vascular tension test were utilized to explore the effects of BK channel deletion on the structure and function changes in skeletal muscle, myocardium, and vascular smooth muscle (VSM). It was found that compared with wild-type rats, the BK-/- rats showed decreased skeletal muscle fiber area, grip, movement distance and speed at 2 and 12 months of ages. At heart, the muscle fiber area, cardiac systolic/diastolic function and heart rate decreased in BK-/- rats. The wall of the left ventricle became thin. However, the vascular wall of BK-/- rats thickened, the pulse wave velocity was increased, and the VSM contraction was enhanced. Unexpectedly, both systolic and diastolic blood pressure were reduced in BK-/- rats, while pulse pressure difference was increased. These results suggest that BK channel may have different effects on different types of muscle tissue, and it should be noted that different parts of muscle tissue may have different effects when BK channel-related drugs are used. (C) 2021 Elsevier Inc. All rights reserved.
