Hypoglycaemic neuropathy: occurrence of axon terminals in plantar skin and plantar muscle of diabetic BB/Wor rats treated with insulin implants
Hypoglycaemic neuropathy: occurrence of axon terminals in plantar skin and plantar muscle of diabetic BB/Wor rats treated with insulin implants
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DOI：
10.1007/pl00007435
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发表时间：
2000-03-01
影响因子：
12.7
通讯作者：
Hildebrand, C
中科院分区：
文献类型：
--
作者：
Mohseni, S;Lillesaar, C;Hildebrand, C
一般认为，糖尿病性神经病变是由于慢性高血压。然而，胰岛素瘤患者的经验和实验研究表明，低血糖也可能导致神经病变。因此，用胰岛素植入物治疗的糖尿病eu-/低血糖BB/Wor大鼠的足底神经表现出明显的神经病变。低血糖性神经病在多大程度上影响皮肤和肌肉中的轴突终末尚不清楚。在本研究中，我们检查发生的表皮轴突档案和神经肽降钙素基因相关肽（CGRP）在足底皮肤，和终板轴突终端在足底肌肉的糖尿病BB/Wor大鼠进行长时间的低血糖。蛋白质基因产物免疫反应性轴突轮廓的数量被发现是正常的，在足跟皮肤活检标本从欧盟/低血糖大鼠，但许多配置文件短而薄。皮肤活检标本中CGRP含量明显低于正常值。用囊泡乙酰胆碱转运蛋白抗体染色后，在EU-/低血糖大鼠的拇短屈肌切片中，终板轴突终末的出现显著减少。此外，终板轴突终末往往是异常小，在这些大鼠。我们的结论是，低血糖神经病变的糖尿病BB/Wor大鼠的足底神经干胰岛素植入剂治疗伴随着轻度的改变，足底皮肤的表皮神经支配和更明显的异常神经末梢模式在足底肌肉。
It is generally believed that diabetic neuropathy is due to chronic hyperglycaemia. However, experience from insulinoma patients and experimental studies show that hypoglycaemia may also cause neuropathy. Accordingly, the plantar nerves of diabetic eu-/hypoglycaemic BB/Wor rats treated with insulin implants exhibit a distinct neuropathy. To what extent hypoglycaemic neuropathy affects axon terminals in skin and muscle is unknown. In the present study we examine the occurrence of epidermal axon profiles and the neuropeptide calcitonin gene-related peptide (CGRP) in plantar skin, and of end plate axon terminals in a plantar muscle of diabetic BB/Wor rats subjected to long periods of hypoglycaemia. The number of protein gene product-immunoreactive axon profiles was found to be normal in heel skin biopsy specimens from eu-/hypoglycaemic rats, but many profiles were short and thin. The content of CGRP in the skin biopsy samples was significantly below normal. After staining with antibodies against the vesicular acetylcholine transporter protein, the occurrence of end plate axon terminals was significantly reduced in sections from the flexor hallucis brevis muscle of eu-/hypoglycaemic rats. Moreover, the end plate axon terminals tended to be abnormally small in these rats. We conclude that the hypoglycaemic neuropathy seen in plantar nerve trunks of diabetic BB/Wor rats treated with insulin implants is accompanied by mild alterations in the epidermal innervation of plantar skin and a more obviously abnormal nerve terminal pattern in plantar muscle.