MUTATIONS IN THE NUCLEAR LAMIN PROTEINS RESULTING IN THEIR ABERRANT ASSEMBLY IN THE CYTOPLASM
MUTATIONS IN THE NUCLEAR LAMIN PROTEINS RESULTING IN THEIR ABERRANT ASSEMBLY IN THE CYTOPLASM
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DOI：
10.1002/j.1460-2075.1988.tb03073.x
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发表时间：
1988-08-01
期刊：
影响因子：
11.4
通讯作者：
MCKEON, F
中科院分区：
文献类型：
--
作者：
LOEWINGER, L;MCKEON, F
我们已经构建了一系列的突变，在人类A核纤层蛋白的cDNA，以确定和改变核定位信号，使用在体内的功能测定系统。核定位信号在核纤层蛋白质具有结构和功能的相似性与SV 40大T抗原。该功能结构域内的突变导致通过细胞质管状结构的组装，并且这些突变体的行为表明核纤层蛋白在其转运到细胞核中之前的翻译后二聚化。在这项工作的过程中，其他地区的羧基末端的A/C核纤层蛋白蛋白质已牵连在正确的组装和结构的核膜。
We have constructed a series of mutations in the human A lamin cDNA to identify and alter the nuclear localization signal using an in vivo functional assay system. The nuclear localization signal in the lamin proteins has both structural and functional similarities with that of the SV40 large T-antigen. Mutations within this functional domain result in the assembly by cytoplasmic tubular structures, and the behavior of these mutants suggests a post-translational dimerization of the lamin proteins prior to their transport into the nucleus. In the course of this work other regions of the carboxy terminus of the A/C lamin proteins have been implicated in the proper assembly and structure of the nuclear envelope.