Heterochromatin integrity affects chromosome reorganization after centromere dysfunction
Heterochromatin integrity affects chromosome reorganization after centromere dysfunction
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DOI：
10.1126/science.1158699
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发表时间：
2008-08-22
期刊：
影响因子：
56.9
通讯作者：
Takahashi, Kohta
中科院分区：
文献类型：
--
作者：
Ishii, Kojiro;Ogiyama, Yuki;Takahashi, Kohta
真核生物染色体上的着丝粒对遗传信息的遗传是必不可少的。着丝粒同一性的表观遗传调控与基因组稳定性、核型进化和物种形成有关。然而，关于着丝粒功能障碍影响染色体结构的方式知之甚少。在这里，我们表明，在裂殖酵母裂殖酵母粟酒裂殖酵母，有条件的删除的着丝粒产生的幸存者，携带要么neocentromere收购染色体在亚端粒区或无着丝粒染色体拯救端粒间融合与其余的染色体。新着丝粒形成与端粒融合的比率通过参与RNA干扰依赖性异染色质形成的基因的失活而显著降低。异染色质的基因组分布可能通过影响染色体重组的方式而影响核型进化的命运。
The centromere is essential for the inheritance of genetic information on eukaryotic chromosomes. Epigenetic regulation of centromere identity has been implicated in genome stability, karyotype evolution, and speciation. However, little is known regarding the manner in which centromere dysfunction affects the chromosomal architectures. Here we show that in the fission yeast Schizosaccharomyces pombe, the conditional deletion of the centromere produces survivors that carry either a neocentromere- acquired chromosome at the subtelomeric region or an acentric chromosome rescued by intertelomere fusion with either of the remaining chromosomes. The ratio of neocentromere formation to telomere fusion is considerably decreased by the inactivation of genes involved in RNA interference- dependent heterochromatin formation. By affecting the modes of chromosomal reorganization, the genomic distribution of heterochromatin may influence the fate of karyotype evolution.