In vivo monitoring of oxidative burst on aloe under salinity stress using hemoglobin and single-walled carbon nanotubes modified carbon fiber ultramicroelectrode
In vivo monitoring of oxidative burst on aloe under salinity stress using hemoglobin and single-walled carbon nanotubes modified carbon fiber ultramicroelectrode
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利用血红蛋白和单壁碳纳米管修饰碳纤维超微电极体内监测盐度胁迫下芦荟的氧化爆发
DOI：
10.1016/j.bios.2013.07.001
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发表时间：
2013-12-15
影响因子：
12.6
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Ren, Qiong-Qiong;Yuan, Xiao-Jun;Chen, Wei
采用单壁碳纳米管 (SWCNT) 和血红蛋白 (Hb) 修饰的碳纤维超微电极 (CFUME) 构建了基于直接电子转移的体内 H2O2 传感器。在-0.1 V的低工作电位下，Hb/SWCNTs/CFUME表现出高达0.405 mM的动态范围，检测下限为4 μM (SIN=3)，灵敏度高达1.07 log(A) log(M)(-1) cm(-2)。表观米氏常数 (K-m, (app)) 估计低至 1.35 mM。由于尺寸极小和工作电位低，Hb/SWCNTs/CFUME 可以直接对盐胁迫下芦荟叶片中的 H2O2 进行 19.5 小时的体内电流监测，无需复杂的数据处理和额外的表面涂层以避免干扰。从12.5 h开始，使用Hb/SWCNTs/CFUME可以清楚地观察到盐胁迫下的芦荟叶片中H2O2水平急剧增加，而在没有盐胁迫的芦荟中，H2O2水平在整个测量过程中保持稳定。为了进一步确认 Hb/SWCNTs/CFUME 的体内响应，将过氧化氢酶 (CAT) 注入传感器附近的位置，导致电流快速下降，这表明 H2O2 被清除。这些结果表明 Hb/SWCNTs/CFUME 可以成为 ROS 体内研究的强大工具。 (C) 2013 Elsevier B.V. 保留所有权利。
Single-walled carbon nanotubes (SWCNTs) and hemoglobin (Hb) modified carbon fiber ultramicroelectrode (CFUME) were employed to construct a direct electron transfer based in vivo H2O2 sensor. At the low working potential of -0.1 V, Hb/SWCNTs/CFUME showed a dynamic range up to 0.405 mM with a low detection limit of 4 mu M (SIN=3) and a high sensitivity of 1.07 log(A) log(M)(-1) cm(-2). The apparent Michaelis-Menten constant (K-m, (app)) was estimated to be as low as 1.35 mM. Due to the extremely small dimension and low working potential, Hb/SWCNTs/CFUME could give directly amperometric in vivo monitoring of H2O2 in aloe leaves with salt stress for 19.5 h without the requirement of complex data processing and extra surface coatings to avoid interferences. The sharp increase of H2O2 level in aloe leaves with salt stress was clearly observed using Hb/SWCNTs/CFUME from 12.5 h, while in the aloe without salt stress, H2O2 level remained stable in the whole measurement. For further confirming the in vivo response of Hb/SWCNTs/CFUME, catalase (CAT) was injected into the spot adjacent to the sensor and caused rapid current decrease, which suggests the scavenging of H2O2. These results indicate that Hb/SWCNTs/CFUME can be a powerful tool for in vivo investigation of ROS. (C) 2013 Elsevier B.V. All rights reserved.