Salinity affects indirectly nitrate acquisition associated with glutamine accumulation in cowpea roots
Salinity affects indirectly nitrate acquisition associated with glutamine accumulation in cowpea roots
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DOI：
10.1007/s10535-012-0065-7
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发表时间：
2012-04
影响因子：
1.5
通讯作者：
J. A. Silveira;A. Melo;M. O. Martins;S. L. Ferreira-Silva;R. Aragão;E. N. Silva;R. A. Viégas
中科院分区：
文献类型：
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作者：
J. A. Silveira;A. Melo;M. O. Martins;S. L. Ferreira-Silva;R. Aragão;E. N. Silva;R. A. Viégas
本研究的目的是验证盐度可以通过谷氨酰胺积累引发的负调节间接影响硝酸盐获取的假设。为了限制豇豆植株的生长和对氮的需求，将豇豆植株暴露在温和的NaCl浓度（50 mM）下。21 d后，预处理植株和对照植株分别在不添加NaCl的情况下分别添加0、5和10 mM的Ca(NO3)2 3 d。盐处理后植株对NO3−的吸收受到很大限制，表现为硝态氮吸收速率、硝态氮积累量、硝态氮还原酶活性和蛋白质含量下降。NO3−利用的限制与谷氨酰胺合成酶活性和谷氨酰胺积累的增加呈正相关，尤其是在根系中。
The aim of this study was to test the hypothesis that salinity can affect indirectly the nitrate acquisition by a negative modulation triggered by glutamine accumulation. Cowpea plants were exposed to a mild NaCl concentration (50 mM) in order to restrict growth and N-demand. After 21 d, pretreated plants and control plants were supplied with 0, 5 and 10 mM of Ca(NO3)2for 3 d in absence of NaCl. Salt pretreated plants showed a great limitation in acquisition of NO3−, indicated by decline in the nitrate uptake rate, NO3−accumulation, nitrate reductase activity and protein content. The restriction of NO3−utilization was positively associated with increased glutamine synthetase activity and glutamine accumulation, especially in roots.