Overexpression of activated CaMKII in the CA1 hippocampus impairs context discrimination, but not contextual conditioning
Overexpression of activated CaMKII in the CA1 hippocampus impairs context discrimination, but not contextual conditioning
复制标题
DOI：
10.1186/s13041-019-0454-3
复制
发表时间：
2019-04-05
期刊：
影响因子：
3.6
通讯作者：
Kaang, Bong-Kiun
中科院分区：
文献类型：
--
作者：
Ye, Sanghyun;Kim, Ji-il;Kaang, Bong-Kiun
钙/钙调蛋白依赖的蛋白激酶II(CaMKII)在记忆形成的分子机制中起着关键作用。已知在记忆形成过程中，CaMKII在激活的脊椎中被特异性地激活。然而，目前还不清楚CaMKII的特定激活是否是编码信息所必需的。在这里，我们在海马CA1区过表达了活性形式的CaMKII(CaMKII*)，以非特异性地激活CaMKII。此外，我们还考察了小鼠的语境辨别能力。我们发现，CaMKII*过度表达的小鼠对语境的辨别能力受到了损害，而语境恐惧条件反射保持完好。这些结果表明，CaMKII激活的空间特异性对于语境辨别是必要的。
Calcium/Calmodulin-dependent protein kinase II (CaMKII) plays a key role in the molecular mechanism of memory formation. CaMKII is known to be activated specifically in the activated spines during memory formation. However, it is unclear whether the specific activation of CaMKII is necessary for encoding information. Here, we overexpressed active form of CaMKII (CaMKII*) in the hippocampal CA1 region to activate CaMKII nonspecifically. Moreover, we examined context-discrimination performance of mice. We found that the mice with overexpression of CaMKII* showed impaired context-discrimination ability, while the contextual fear conditioning remained intact. These results indicate that spatial specificity of CaMKII activation is necessary for context discrimination.