Membrane-Directed High Bactericidal Activity of (Gold Nanoparticle)Polythiophene Composite for Niche Applications Against Pathogenic Bacteria
Membrane-Directed High Bactericidal Activity of (Gold Nanoparticle)Polythiophene Composite for Niche Applications Against Pathogenic Bacteria
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DOI：
10.1002/adhm.201200278
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发表时间：
2013-04-01
影响因子：
10
通讯作者：
Ramesh, Aiyagari
中科院分区：
文献类型：
--
作者：
Adhikari, Manab Deb;Goswami, Sudeep;Ramesh, Aiyagari
纳米材料作为杀菌剂的使用代表了针对耐药病原菌的治疗剂开发的新范例。本文报道了一种水溶性（金纳米颗粒）聚噻吩（AuNP-PTh）复合物对常见细菌病原体的抗菌活性。该纳米复合材料具有广谱的杀菌活性，并对靶病原体表现出膜导向的作用模式。通过实验证明了AuNP-PTh的治疗效力，这些实验揭示了纳米复合物可以突破革兰氏阴性病原体的外膜防御屏障，用于随后被疏水抗生素杀死，抑制模拟胃液中模型胃肠道病原体的生长，并显著消除细菌生物膜。高细菌选择性和对人细胞缺乏细胞毒性很好地促进了纳米复合材料针对临床相关病原菌的未来治疗应用。
The use of nanoscale materials as bactericidal agents represents a novel paradigm in the development of therapeutics against drug-resistant pathogenic bacteria. In this paper the antimicrobial activity of a water soluble (gold nanoparticle)polythiophene (AuNP-PTh) composite against common bacterial pathogens is reported. The nanocomposite is broad-spectrum in its bactericidal activity and exhibits a membrane-directed mode of action on target pathogens. The therapeutic potency of AuNP-PTh is demonstrated by experiments which reveal that the nanocomposite can breach the outer membrane defense barrier of Gram-negative pathogens for subsequent killing by a hydrophobic antibiotic, inhibit the growth of model gastrointestinal pathogens in simulated gastric fluid, and significantly eradicate bacterial biofilms. The high bacterial selectivity and lack of cytotoxicity on human cells augers well for future therapeutic application of the nanocomposite against clinically relevant pathogenic bacteria.