Periodic gene expression program of the fission yeast cell cycle
Periodic gene expression program of the fission yeast cell cycle
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DOI：
10.1038/ng1377
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发表时间：
2004-08-01
期刊：
影响因子：
30.8
通讯作者：
Bähler, J
中科院分区：
文献类型：
--
作者：
Rustici, G;Mata, J;Bähler, J
细胞周期调控转录似乎是普遍的，但很少有人知道它的全球保护和生物学意义。我们报告了裂殖酵母细胞周期的全基因组转录程序，确定了407个周期性表达的基因，其中136个显示出高幅度的变化。这些基因聚集在四个主要的表达波中。叉头蛋白Sep 1 p调节第一簇中的有丝分裂基因，包括Ace 2 p，Ace 2 p在M-G1转换和胞质分裂期间激活第二簇中的转录。第二簇中的其他基因是G1-S进程所需的，由MBF复合物独立于Sep 1 p和Ace 2 p调节。第三个簇与S期一致，第四个簇包含G2期弱调控的基因。尽管保守的细胞周期转录因子，在调节电路之间的差异裂变和芽殖酵母是显而易见的，揭示了转录控制的进化可塑性。大多数基因的周期性转录在两种酵母之间并不保守，除了一组类似于40个基因的核心，这些基因似乎在真核细胞周期中受到普遍调控，并可能在细胞周期进程中起关键作用。
Cell-cycle control of transcription seems to be universal, but little is known about its global conservation and biological significance. We report on the genome-wide transcriptional program of the Schizosaccharomyces pombe cell cycle, identifying 407 periodically expressed genes of which 136 show high-amplitude changes. These genes cluster in four major waves of expression. The forkhead protein Sep1p regulates mitotic genes in the first cluster, including Ace2p, which activates transcription in the second cluster during the M-G1 transition and cytokinesis. Other genes in the second cluster, which are required for G1-S progression, are regulated by the MBF complex independently of Sep1p and Ace2p. The third cluster coincides with S phase and a fourth cluster contains genes weakly regulated during G2 phase. Despite conserved cell-cycle transcription factors, differences in regulatory circuits between fission and budding yeasts are evident, revealing evolutionary plasticity of transcriptional control. Periodic transcription of most genes is not conserved between the two yeasts, except for a core set of similar to40 genes that seem to be universally regulated during the eukaryotic cell cycle and may have key roles in cell-cycle progression.