Prevalence of latent tuberculosis infection in German radiologists
Prevalence of latent tuberculosis infection in German radiologists
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DOI：
10.1016/j.jhin.2008.02.009
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发表时间：
2008-05-01
影响因子：
6.9
通讯作者：
Lindemann, M.
中科院分区：
文献类型：
--
作者：
Barsegian, V.;Mathias, K. D.;Lindemann, M.
与一般人群相比，卫生保健工作者感染结核病的风险增加。由于目前结核菌素皮肤试验（TST）的不足，很少有人尝试量化德国医护人员中潜伏性结核病感染的患病率。因此，我们的目的是使用结核病特异性ELISpot（T-SPOT（R）.TB）检测来调查该队列中潜伏性结核病的患病率，并比较该检测与TST的性能。对95名放射科健康体检者进行了ELISpot、淋巴细胞转化试验和TST检测。对于细胞体外试验，使用结核特异性肽和纯化的蛋白衍生物（PPD）作为抗原。这些测试与先前结核病暴露的问卷调查相结合。在95名医务人员中，只有1名（1%）被T-SPOT.TB、92名（97%）被PPD-ELISpot、78名（82%）被PPD-淋巴细胞转化试验和32名（34%）被TST确定为阳性。多变量分析显示，TST受外来出生和既往皮肤试验的显著影响（分别为P < 0.0001和P = 0.001）。T-SPOT.TB检测结果与外国出生、既往皮肤检测和既往结核病疫苗接种无关。与TST相比，T-SPOT.TB似乎是追踪这一高危群体结核病感染的准确且有用的工具。95名参与者中只有一人患有潜伏性结核病，这些初步结果表明德国放射科医生的潜伏性结核病发病率较低。(c)2008医院感染协会。由Elsevier Ltd.出版。保留所有权利。
Healthcare workers have an increased risk of tuberculosis infection compared with the general population. There have been few attempts to quantify the prevalence of latent tuberculosis infection amongst German healthcare workers, due to inadequacy of the current tuberculin skin test (TST). Therefore, it was our aim to investigate the prevalence of latent tuberculosis in this cohort using a tuberculosis-specific ELISpot (T-SPOT (R).TB) test and to compare the performance of this test to that of the TST. Ninety-five healthy participants working in departments of radiology were examined by ELISpot, lymphocyte transformation test and TST. For cellular in-vitro tests, tuberculosis-specific peptides and purified protein derivate (PPD) were used as antigens. These tests were combined with a questionnaire on prior tuberculosis exposure. Out of 95 healthcare workers, only one (1%) was defined as positive by T-SPOT.TB, 92 (97%) by PPD-ELISpot, 78 (82%) by PPD-lymphocyte transformation test and 32 (34%) by TST. Multivariate analysis showed that the TST was significantly affected (P < 0.0001 and P = 0.001, respectively) by foreign birth and prior skin testing. The T-SPOT.TB test results were independent of foreign birth, prior skin testing and prior vaccination against tuberculosis. In contrast to the TST, T-SPOT.TB appears to be an accurate and useful tool to track tuberculosis infection in this at-risk group. With only one of 95 participants having acquired latent tuberculosis, these preliminary results argue for a low incidence of latent tuberculosis in German radiologists. (c) 2008 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.