COMPARISON OF THE GROWTH OF NORMAL AND NEOPLASTIC MOUSE MAMMARY CELLS ON PLASTIC, ON COLLAGEN GELS AND IN COLLAGEN GELS
COMPARISON OF THE GROWTH OF NORMAL AND NEOPLASTIC MOUSE MAMMARY CELLS ON PLASTIC, ON COLLAGEN GELS AND IN COLLAGEN GELS
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DOI：
10.1016/0014-4827(83)90319-1
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发表时间：
1983-01-01
影响因子：
3.7
通讯作者：
NANDI, S
中科院分区：
文献类型：
--
作者：
RICHARDS, J;PASCO, D;NANDI, S
在塑料、大鼠尾胶原凝胶和大鼠尾胶原凝胶上的原代培养物中比较正常和肿瘤小鼠乳腺细胞的生长。塑料上的细胞在最初几天生长，然后在培养2周后停止生长，细胞数量仅增加1至3倍。在胶原凝胶上或胶原凝胶中生长的细胞在2周的培养期内连续生长，胶原凝胶上培养的细胞数增加高达10倍，包埋在胶原凝胶中的细胞增加20倍。解释了在胶原凝胶中生长的细胞和在胶原凝胶上生长的细胞之间的生长速率的差异。在胶原凝胶上和胶原凝胶中生长的细胞可以产生电子致密的基底层粘连样结构，其与胶原IV和层粘连蛋白相关，如通过免疫荧光所判断的。在塑料上生长的细胞不会形成这种结构。顺式-OH-脯氨酸阻断胶原蛋白的产生并抑制培养细胞的生长，表明胶原蛋白的产生参与生长。鼠尾胶原凝胶是小鼠乳腺细胞生长的上级基质，这可能与细胞形成含IV型胶原的基底层样结构的能力有关。
The growth of normal and neoplastic mouse mammary cells was compared in primary cultures on plastic, on rat tail collagen gels and in rat tail collagen gels. Cells on plastic grew for the first few days, then stopped with only a 1- to 3-fold increase in cell number after 2 wk in culture. Cells grown on or in collagen gels grew continuously over the 2-wk culture period with up to 10-fold increase in cell number of cultures on collagen gels and a 20-fold increase for cells embedded in collagen gels. The difference in growth rates between cells grown in collagen gel and those grown on collagen gels was explained. Cells grown on and in collagen gel can produce an electron-dense basal lamina-like structure which is associated with collagen IV and laminin as judged by immunofluorescence. Cells grown on plastic do not form this structure. Cis-OH-proline blocks the production of collagen and inhibits the growth of the cultured cells, indicating collagen production to be involved in growth. Rat tail collagen gels are a superior substratum for the growth of mouse mammary cells and this may be related to the cells'' ability to form a collagen IV-containing basal lamina-like structure.