PEROXYNITRITE CAUSES DNA NICKS IN PLASMID PBR322
PEROXYNITRITE CAUSES DNA NICKS IN PLASMID PBR322
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DOI：
10.1006/bbrc.1995.1759
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发表时间：
1995-05-25
影响因子：
3.1
通讯作者：
PRYOR, WA
中科院分区：
文献类型：
--
作者：
SALGO, MG;STONE, K;PRYOR, WA
琼脂糖凝胶电泳分析证明过氧亚硝酸盐导致pBR 322超螺旋DNA单链断裂。研究了甘露醇、苯甲酸盐和二甲基亚砜三种自由基清除剂的作用。甘露醇未能保护DNA免受过氧亚硝酸盐的损伤，而苯甲酸盐和二甲亚砜则放大了损伤。这些结果表明，过氧亚硝酸盐单独引起的损伤不是由自由基介导的，因为典型的自由基清除剂不能防止损伤。(C)出版社：Academic Press
Peroxynitrite causes single-strand breaks in pBR322 supercoiled DNA as evidenced by agarose gel electrophoresis analysis. The effect of three free radical scavengers, namely mannitol, benzoate and dimethylsulfoxide, were studied. Mannitol failed to protect DNA from damage by peroxynitrite while benzoate and dimethylsulfoxide amplified the damage. These results suggest the damage caused by peroxynitrite alone is not mediated by free radicals since typical free radical scavengers fail to prevent the damage. (C) 1995 Academic Press,Inc.