Antioxidant activity of an exopolysaccharide isolated from Lactobacillus plantarum C88.
Antioxidant activity of an exopolysaccharide isolated from Lactobacillus plantarum C88.
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从植物乳杆菌 C88 中分离的胞外多糖的抗氧化活性。
DOI：
10.1016/j.ijbiomac.2012.12.037
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发表时间：
2013-03
期刊：
影响因子：
--
通讯作者：
Li Zhang
中科院分区：
文献类型：
--
作者：
Li Zhang
从植物乳杆菌（Lactobacillus plantarum）C88中分离得到一种中性胞外多糖（EPS），命名为LPC-1。LPC-1的平均分子量为1.15× 106 Da，由半乳糖和葡萄糖组成，摩尔比为1：2。通过体外清除羟自由基和1，1-二苯基-2-苦肼基（DPPH）自由基的能力评价LPC-1的抗氧化活性。结果表明，LPC-1对羟自由基有较好的清除能力。进一步研究LPC-1对H2 O2诱导的Caco-2细胞氧化损伤的保护作用。结果表明，LPC-1能抑制丙二醛（MDA）的生成，提高超氧化物歧化酶（SOD）活性和总抗氧化能力（T-AOC），且呈剂量依赖性。这些结果表明L.植物C88具有抗氧化作用，其可能涉及清除活性氧（ROS）、上调酶和非酶抗氧化活性以及减少脂质过氧化。
A neutral exopolysaccharide (EPS), designated LPC-1, was isolated from the culture of Lactobacillus plantarum C88 and purified by ion-exchange and gel-permeation chromatography. LPC-1 had an average molecular weight of 1.15×106Da, and it was composed of galactose and glucose with a molar ratio of 1:2. The antioxidant activity of LPC-1 was evaluated with the in vitro scavenging abilities on hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The results indicated that LPC-1 had good scavenging ability on hydroxyl radicals. Furthermore, the protective effect of LPC-1 on H2O2-induced Caco-2 cells oxidative injury was investigated. As results, LPC-1 inhibited the formation of malondialdehyde (MDA) and raised the activities of superoxide dismutase (SOD) and total antioxidant capacities (T-AOC) in a dose-dependent manner. These results demonstrate that the EPS from L. plantarum C88 has antioxidant effects that may involve scavenging of reactive oxygen species (ROS), up-regulation of enzymatic and non-enzymatic antioxidant activities, and reduction of lipid peroxidation.
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