New Chemiluminescence Detection Method of Aliphatic Conjugated Dienes Using Electrogenerated Tris(2,2′‐Bipyridine)Ruthenium(III)
New Chemiluminescence Detection Method of Aliphatic Conjugated Dienes Using Electrogenerated Tris(2,2′‐Bipyridine)Ruthenium(III)
复制标题
电化学发光三(2,2′-联吡啶)钌(III)检测脂肪族共轭二烯的新方法
DOI：
10.1081/al-200045155
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发表时间：
2005
影响因子：
2
通讯作者：
A. Muromatsu
中科院分区：
文献类型：
--
作者：
Hitoshi Kodamatani;Keiitsu Saito;Nobumitsu Niina;S. Yamazaki;A. Muromatsu
摘要在合适的还原剂作用下，三(2，2‘-联吡啶)Ru(III)发生化学发光反应。具有共轭双键的有机化合物，如2，4-己二烯和α-松油烯，在pH为3到7的范围内进行了测试，发现它们作为化学发光还原剂。脂肪族共轭双烯的化学发光强度依赖于进行化学发光反应的pH，并且在酸性水溶液中观察到强烈的化学发光。结果表明，碳原子共轭双键上官能团的吸电子/给电子特性影响反应的化学发光效率。基于化学发光反应，建立了一种测定脂肪族共轭双烯的新方法。在信噪比为3的情况下，2，5-二甲基-2，4-己二烯的最低检测下限为0.6pmol。这项工作(部分)得到了日本科学学会Sasakawa科学研究基金的支持。
Abstract Tris(2,2′‐bipyridine)ruthenium(III) will undergo a chemiluminescent reaction with an appropriate reducing agent. Organic compounds with conjugated double bonds such as 2,4‐hexadiene and α‐terpinene were tested between a pH of 3 and 7 and found to act as chemiluminescent reducing agents. The chemiluminescent intensities for aliphatic conjugated dienes were dependent upon the pH at which the chemiluminescence reaction was conducted, and intense chemiluminescence was observed in acidic aqueous solutions. It has been shown that the electron‐withdrawing/donating character of the functional group attached to the carbon atoms building conjugated double bonds influences the chemiluminescent efficiency of the reaction. A novel chromatographic method for the determination of aliphatic conjugated dienes based on the chemiluminescent reaction was developed. The lowest detection limit of 0.6 pmol in high‐performance liquid chromatography was found for 2,5‐dimethyl‐2,4‐hexadiene at a signal‐to‐noise ratio of 3. This work (partly) was supported by the Sasakawa Scientific Research Grant from The Japan Science Society.