NUMBERS OF NERVE FIBERS IN VAGUS NERVE OF MAN
NUMBERS OF NERVE FIBERS IN VAGUS NERVE OF MAN
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DOI：
10.1002/ar.1091390312
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发表时间：
1961-01-01
影响因子：
--
通讯作者：
SCHNITZLEIN, HN
中科院分区：
文献类型：
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作者：
HOFFMAN, HH;SCHNITZLEIN, HN
材料与方法在17具身体标本上，切断颈中迷走神经、喉返神经，切断所有肉眼可见的胸支，切除横隔部的食道迷走神经。在这项研究中所报道的神经是那些在更早的文献(Schnitzlein，Rowe and Hoffman，‘58)中报道的神经的近端。这项研究中应用的神经节段切除的限制同样适用于本研究中报道的那些。本文报道的计数是在吡啶-银浸渍技术之后进行的，在此过程中，它们被金色染色，并用Feulgen、坚固绿色和橙色G进行反染(Foley，‘38)。采用眼格估计法(Schnitzlein and Foley，‘57)对纤维进行计数。所使用的显微镜配备了油浸物镜(120X，NA 1.3)和15X望远镜，以获得足够的分辨率和放大倍率。几乎所有报告的统计数字都代表了至少两个独立估计的平均值。应该注意的是，一些交感神经束可能与所有水平的迷走神经一起走行。这些纤维束通常很容易辨认，特别是在宫颈中部的吡啶-银制剂中，并已尽可能地从计数中消除。
MATERIALS AND METHODSSegments of mid-cervical vagi, recurrent laryngeal nerves after all the grossly observable thoracic branches have been given off, and the esophageal vagi at the diaphragm were removed at 17 autopsies. The nerves reported in this study are adja-cent segments just proximal to those re-ported in an earlier publication (Schnitzlein, Rowe and Hoffman,'58). The same limitations which applied to the removal of the nerve segments utilized in the myehated study applies to those reported in this investigation.The counts reported here were made fol-lowing a pyridine-silver impregnation technique in the course of which they were gold toned and counterstained with Feulgen, fast green and orange G (Foley,'38). An ocular-grid estimation technique (Schnitzlein and Foley,'57) was used to enumerate the fibers. The microscopes employed were equipped with oil-immersion objectives (120 X, NA 1.3) and 15 X oculars in order to obtain adequate resolution and magnification. Nearly all reported counts represent an average of at least two independent estimations. It should be noted that some sympathetic fascicles may possibly course with the vagus nerve at all levels. These fascicles are usually readily recognizable, especially at mid-cervical levels in the pyridine-silver preparations, and have been eliminated as far as possible from the counts.