Arachidonic acid supplementation does not affect N-methyl-N-nitrosourea-induced renal preneoplastic lesions in young Lewis rats
Arachidonic acid supplementation does not affect N-methyl-N-nitrosourea-induced renal preneoplastic lesions in young Lewis rats
复制标题
花生四烯酸补充剂不会影响年轻 Lewis 大鼠中 N-甲基-N-亚硝基脲诱导的肾癌前病变
作者：
K. Yoshizawa;Y. Emoto;Y. Kinoshita;Ayako Kimura;N. Uehara;T. Yuri;N. Shikata;T. Hamazaki;A. Tsubura
花生四烯酸（AA）天然存在于人类母乳中。AA和二十二碳六烯酸，根据国际食品法典标准，通常作为功能性食品成分添加到世界各地的商业婴儿配方奶粉中。然而，很少有研究表明，在新生儿时期补充AA可能会导致肾脏致癌。研究了妊娠期和哺乳期饲粮中添加AA对n -甲基-n -亚硝基脲（MNU）诱导的幼年Lewis大鼠肾脏肿瘤前病变的影响。饲喂AA含量为2.0%的饲粮或AA含量<0.01%的基础饲粮。出生时（出生后第0天），雄性和雌性幼崽接受单次腹腔注射35 mg/kg MNU或对照物。分别于第7、14、21、28、60天进行肾脏形态学检查。在组织病理学上，两组在第60天均发现肾肿瘤前病变，如肾母细胞瘤病和间充质细胞增殖。基础日粮组和AA日粮组的病变发生率分别为38%和31%，差异无统计学意义。综上所述，在妊娠期和哺乳期喂养富含aa的母鼠不会改变母鼠后代的mnu诱导的肾脏肿瘤前病变。
Arachidonic acid (AA) is naturally found in human breast milk. AA, together with docosahexaenoic acid, is commonly added as a functional food ingredient to commercial infant formula worldwide, in accordance with the international standards of Codex Alimentarius. However, few studies of the possible renal carcinogenic effects of AA supplementation during neonatal life have been performed. The effect of dietary AA supplementation in dams during gestation and lactation was investigated on N-methyl-N-nitrosourea (MNU)-induced preneoplastic lesions in the kidneys of young Lewis rats. Dams were fed a 2.0% AA diet or a basal diet (<0.01% AA). At birth (postnatal day 0), male and female pups received a single intraperitoneal injection of 35 mg/kg MNU or vehicle. Renal morphology was examined after 7, 14, 21, 28 and 60 days. Histopathologically, renal preneoplastic lesions, such as nephroblastomatosis and mesenchymal cell proliferation, were found on day 60 in both the MNU-treated groups. There was no significant difference in lesion incidence of 38% in the basal diet group and 31% in the AA diet group. In conclusion, an AA-rich diet for dams during gestation and lactation does not modify MNU-induced renal preneoplastic lesions in their offspring.