Intra- and interspecific comparisons of bacterial diversity and community structure support coevolution of gut microbiota and termite host
Intra- and interspecific comparisons of bacterial diversity and community structure support coevolution of gut microbiota and termite host
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DOI：
10.1128/aem.71.11.6590-6599.2005
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发表时间：
2005-11-01
影响因子：
4.4
通讯作者：
Kudol, T
中科院分区：
文献类型：
--
作者：
Hongoh, Y;Deevong, P;Kudol, T
采用16S rRNA的末端限制性片段长度多态性分析和克隆分析方法，对4种微角白蚁(白蚁科)和4种白蚁(雷白蚁科)共32个木饲白蚁种群的肠道微生物区系进行了研究。对所获得的白蚁分子群落图谱进行了个体、群体、地点和种类的统计比较。两种分析都表明，白蚁各属的细菌群落结构非常相似，采样点和/或白蚁种类之间的差异很小，但差异很大。相反，两个白蚁属之间存在较大差异。两个白蚁属之间只有一个细菌系统型(97%的序列同源性)，而小白蚁属和白蚁属的两个同属种之间分别有18%和50%的系统型相同。同时对微白蚁属228个系统发育类型进行了系统发育分析。和367个种的系统模式。与公共数据库中提供的其他白蚁肠道克隆一起，显示了许多与白蚁远亲有关的系统类型的单系性。由一个以上白蚁种的系统类型组成的单系白蚁簇分布在15个细菌门中，包括新的候选门TG2和TG3。在本研究分析的960个无性系中，这些白蚁聚集物占95%。此外，12门的聚类群由一个以上的白蚁(亚科)科组成，占分析无性系的75%。我们的结果表明，大多数肠道细菌不是异地的，而是与白蚁共同进化的特定共生体，它们的群落结构在白蚁属内基本一致。
We investigated the bacterial gut microbiota from 32 colonies of wood-feeding termites, comprising four Microcerotermes species (Termitidae) and four Reticulitermes species (Rhinotermitidae), using terminal restriction fragment length polymorphism analysis and clonal analysis of 16S rRNA. The obtained molecular community profiles were compared statistically between individuals, colonies, locations, and species of termites. Both analyses revealed that the bacterial community structure was remarkably similar within each termite genus, with small but significant differences between sampling sites and/or termite species. In contrast, considerable differences were found between the two termite genera. Only one bacterial phylotype (defined with 97% sequence identity) was shared between the two termite genera, while 18% and 50% of the phylotypes were shared between two congeneric species in the genera Microcerotermes and Reticulitermes, respectively. Nevertheless, a phylogenetic analysis of 228 phylotypes from Microcerotermes spp. and 367 phylotypes from Reticulitermes spp. with other termite gut clones available in public databases demonstrated the monophyly of many phylotypes from distantly related termites. The monophyletic "termite clusters" comprised of phylotypes from more than one termite species were distributed among 15 bacterial phyla, including the novel candidate phyla TG2 and TG3. These termite clusters accounted for 95% of the 960 clones analyzed in this study. Moreover, the clusters in 12 phyla comprised phylotypes from more than one termite (sub)family, accounting for 75% of the analyzed clones. Our results suggest that the majority of gut bacteria are not allochthonous but are specific symbionts that have coevolved with termites and that their community structure is basically consistent within a genus of termites.