Parathyroid ultrastructure after aldehyde fixation, high-pressure freezing, or microwave irradiation.
Parathyroid ultrastructure after aldehyde fixation, high-pressure freezing, or microwave irradiation.
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乙醛固定、高压冷冻或微波照射后的甲状旁腺超微结构。
DOI：
10.1177/35.12.3680934
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
H. Moor
中科院分区：
文献类型：
--
作者：
Rolf Marti;P. Wild;E. Schraner;Martin Mueller;H. Moor
甲状旁腺细胞变异体，通常在戊二醛浸泡固定的甲状旁腺中观察到，被认为是甲状旁腺激素分泌过程中周期性变化的结果。将牛甲状旁腺浸入由1%戊二醛、1.5%甲醛和2.5%丙烯醛组成的混合物中，然后在1%四氧化锇中进行后固定，导致仅一种细胞变体的高度均匀性，而相同的固定程序导致大鼠甲状旁腺细胞膜破裂和细胞变体的形成。牛和大鼠的甲状旁腺制备的高压速冻和随后的冷冻置换只包含一个细胞变体。牛和大鼠甲状旁腺的超微结构的良好保存，也表现出只有一个细胞的变种，实现了微波照射的存在下，2.5%的戊二醛在钠-cacodylate后固定与OsO 4在钠-cacodylate或S-可力丁，都含有Ca 2+和Mg 2+。使用适当的缓冲液以及渗透对于利用微波能量成功固定至关重要。主要影响被认为是在足够短的时间内加热标本和增强随后的锇固定。结果支持的想法，产生后检查灌注固定的甲状旁腺组织，甲状旁腺细胞的变异发生在不正确的醛固定，而不是他们表达功能的多样性。
Parathyroid cell variants, commonly observed in parathyroid glands fixed by immersion in glutaraldehyde, are believed to be the result of cyclic changes in the course of parathyroid hormone secretion. Immersion of bovine parathyroid glands in a mixture consisting of 1% glutaraldehyde, 1.5% formaldehyde, and 2.5% acrolein, followed by post-fixation in 1% osmium tetroxide, resulted in high uniformity with only one cell variant, whereas the same fixation procedure led to disruption of cell membranes and formation of cell variants in rat parathyroids. Parathyroid glands of both cattle and rats prepared by high-pressure quick-freezing and subsequent freeze-substitution contained only one cell variant. Excellent preservation of the ultrastructure of bovine and rat parathyroids, also exhibiting only one cell variant, was achieved by microwave irradiation in the presence of 2.5% glutaraldehyde in Na-cacodylate followed by post-fixation with OsO4 in Na-cacodylate or s-collidine, both containing Ca2+ and Mg2+. Use of the appropriate buffer, as well as osmication, is essential for successful fixation utilizing microwave energy. The main effects are considered to be heating specimens within sufficient short periods and enhancement of subsequent osmium fixation. The results support the idea, arising after examination of perfusion-fixed parathyroid tissue, that parathyroid cell variants occur during improper aldehyde fixation rather than that they express functional diversity.
电子显微镜的微波能量固定。
DOI：
--
发表时间：
1985
期刊：
The American journal of pathology
影响因子：
--
作者：
Login,GR;Dvorak,AM
通讯作者：
Dvorak,AM
用于电子显微镜的超快微波能量固定。
DOI：
10.1177/34.3.3950387
发表时间：
1986
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Login,GR;Stavinoha,WB;Dvorak,AM
通讯作者：
Dvorak,AM