Construction of a high-density genetic linkage map and QTL mapping of growth and cold tolerance traits in tiger puffer Takifugu rubripes
Construction of a high-density genetic linkage map and QTL mapping of growth and cold tolerance traits in tiger puffer Takifugu rubripes
复制标题
DOI：
10.1016/j.aquaculture.2022.738613
复制
发表时间：
2022-08-04
期刊：
影响因子：
4.5
通讯作者：
Sun, Zhibin
中科院分区：
文献类型：
--
作者：
Liu, Zhifeng;Wang, Xinan;Sun, Zhibin
虎河豚（Takifugu rubripes）是一种重要的水产养殖品种，其耐低温育种对产业具有积极影响。然而，该物种的遗传改良仍处于起步阶段。为了促进老虎河豚的育种进程，构建遗传连锁图谱，鉴定和验证与性状相关的分子标记是进行标记辅助选择（markerassisted selection， MAS）的第一步。本研究通过全基因组重测序，构建了虎河豚高密度遗传连锁图谱。该图谱由4416个bin标记组成，分布在22个连锁群（LGs）上，总遗传盖度为3147.8 cM，平均密度为0.76 cM。在此基础上，数量性状位点（QTL）定位结果显示，5个LGs在全基因组显著阈值上鉴定出14个与体重、体长和全长相关的QTL，解释了11.1% ~ 16.7%的表型变异。其中10个qtl位于LG3，与体重相关的qtl均位于LG3。有4个重叠区域的qtl与体重和总长度相关。此外，本研究首次完成了老虎河豚抗寒性状的QTL分析，结果表明，在6个LGs中鉴定出8个QTL，解释了13.6 ~ 16.3%的表型变异。本研究获得的重要经济性状的连锁图谱和QTL为老虎河豚的遗传研究和分子标记辅助育种提供了有用的工具。
The tiger puffer (Takifugu rubripes) is an important aquaculture species, and its low temperature tolerance breeding has a positive impact on the industry. However, genetic improvement of the species is still in its infancy. In order to promote the breeding process of tiger puffer, the construction of a genetic linkage map and the identification and validation of molecular markers associated with traits are the first steps towards markerassisted selection (MAS). In this study, a high-density genetic linkage map of the tiger puffer was constructed by whole-genome resequencing. The map consisted of 4416 bin markers distributed over 22 linkage groups (LGs) with a total genetic coverage of 3147.8 cM and an average density of 0.76 cM. On this basis, quantitative trait locus (QTL) mapping results revealed that 14 QTLs associated with body weight, body length and total length were identified in five LGs at the genome-wide significance threshold, explaining 11.1-16.7% of the phenotypic variation. Among them, 10 QTLs were located in LG3, with the QTLs associated with body weight all located in LG3. There were four QTLs with overlapping regions which were associated with both body weight and total length. In addition, this study completed the QTL analysis of cold resistance traits of tiger puffer for the first time, and the results showed that eight QTLs were identified in six LGs, which explained 13.6-16.3% of the phenotypic variation. The linkage map and QTL for important economic traits obtained in this study provide useful tools for genetic research and molecular marker-assisted breeding of tiger puffer.