A microsatellite marker based framework linkage map of Vitis vinifera L.
A microsatellite marker based framework linkage map of Vitis vinifera L.
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DOI：
10.1007/s00122-003-1488-5
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发表时间：
2004-03-01
影响因子：
5.4
通讯作者：
Meredith, CP
中科院分区：
文献类型：
--
作者：
Riaz, S;Dangl, GS;Meredith, CP
利用微卫星标记构建了葡萄属葡萄属的连锁框架图，欧洲酿酒葡萄作图群体由153个来自葡萄属vinifera cvs杂交的后代植株组成。雷司令x赤霞珠。将152个微卫星标记和1个多态性EST标记定位到20个连锁群（2n=38）。该地图覆盖1，728厘米，标记之间的平均距离为11.0厘米。用135-140个标记确定基因组大小的估计值、预期的基因组覆盖率和观察到的基因组覆盖率。父亲和母亲的数据集之间的基因组长度估计不同。观察到的近似基因组覆盖率为65%，而预期覆盖率为90%。减数分裂重组率没有显着差异之间的母亲和父亲的父母。该图谱已被国际葡萄基因组计划采纳为参考图谱。
We have constructed a framework linkage map based on microsatellite markers for Vitis vinifera L., the European wine grape. The mapping population consisted of 153 progeny plants from a cross of Vitis vinifera cvs. Riesling x Cabernet Sauvignon. One hundred fifty-two microsatellite markers and one polymorphic EST marker have been mapped to 20 linkage groups (2n=38). The map covers 1,728 cM with an average distance between markers of 11.0 cM. Estimates of genome size, expected genome coverage, and observed genome coverage were determined with 135-140 markers. Genome length estimates differed between paternal and maternal data sets. Observed approximate genome coverage was 65% versus an expected coverage of 90%. Meiotic recombination rates were not significantly different between maternal and paternal parents. This map has been adopted as a reference map for the International Grape Genome Program.