Myb protein specificity: evidence of a context-specific transcription factor code
Myb protein specificity: evidence of a context-specific transcription factor code
复制标题
DOI：
10.1016/s1079-9796(03)00151-7
复制
发表时间：
2003-09-01
影响因子：
2.3
通讯作者：
Ness, SA
中科院分区：
文献类型：
--
作者：
Ness, SA
脊椎动物表达三种不同的Myb家族转录因子，a -Myb， B-Myb和c-Myb，它们共享一个高度保守的DNA结合域，结合相同的DNA序列，并在转染试验中激活相同的报告基因构建。然而，这三种Myb蛋白具有完全不同的生物学作用，微阵列分析表明，每种蛋白的异位表达引起不同组人类基因的激活。此外，在不同的细胞类型中，被每种蛋白激活的基因是不同的，这表明Myb转录因子受环境特异性调节机制的影响。这些数据支持一种修饰代码模型，其中Myb蛋白在不同的细胞类型中被不同的翻译后修饰修饰，这些修饰控制着Myb蛋白与激活特定基因集所需的其他细胞成分相互作用的能力。(C) 2003 Elsevier Inc.版权所有。
Vertebrates express three different Myb family transcription factors, A-Myb, B-Myb, and c-Myb, that share a highly conserved DNA binding domain, bind to the same DNA sequences, and activate the same reporter gene constructs in transfection assays. However, the three Myb proteins have completely different biological roles, and microarray assays have shown that ectopic expression of each protein causes the activation of different sets of human genes. Furthermore, the genes that are activated by each protein in different cell types are distinct, suggesting that Myb transcription factors are subject to context-specific regulatory mechanisms. The data support a modification code model in which Myb proteins become decorated with different sets of posttranslational modifications in different cell types, and these modifications control the ability of the Myb proteins to interact with other cellular components that are required for activating specific sets of genes. (C) 2003 Elsevier Inc. All rights reserved.