The UK Biobank sample handling and storage validation studies
The UK Biobank sample handling and storage validation studies
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DOI：
10.1093/ije/dyn019
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发表时间：
2008-04-01
影响因子：
7.7
通讯作者：
Elliott, Paul
中科院分区：
文献类型：
--
作者：
Peakman, Tim C.;Elliott, Paul
背景和目的英国生物库是英国的一项大型前瞻性研究，旨在调查遗传因素，环境暴露和生活方式在晚期和中年主要疾病原因中的作用。这项计划包括收集五十万名年龄介乎四十至六十九岁人士的血液及尿液。如何收集、处理和储存样本将对英国生物库资源未来的科学用途产生重大影响。方法收集40名健康志愿者的血液和尿液样本，按照标准处理或在规定温度下保存（所有试管均为4 ℃，但含有酸性柠檬酸盐葡萄糖的真空采血管除外，其维持在18 ℃），处理前24或36 h。将另一个样品在4 ℃下保持24小时，处理并将等分试样在-80 ℃下冷冻20天，然后在受控条件下解冻。结果在4 ℃条件下，样品在采集后24 h内稳定，在不同的检测条件下，样品在不同的时间内稳定。在代谢组学研究中，在4 ℃下保持36小时的样品中观察到小但显著的变化。在受控条件下短期冻融后，一系列生化测定的样品均未发生降解。全血保持在18摄氏度的vacutainers含有酸柠檬酸葡萄糖24小时是适合于病毒immortalization techniques.Conclusions在此补充报告的验证研究提供的理由，通过在英国生物库项目的样品处理和储存程序。
Background and aims UK Biobank is a large prospective study in the United Kingdom to investigate the role of genetic factors,environmental exposures and lifestyle in the causes of major diseases of late and middle age. it involves the collection of blood and urine from 500000 individuals aged between 40 and 69 years. How the samples are collected, processed and stored will have a major impact on the future scientific usefulness of the UK Biobank resource. A series of validation studies was recommended to test the robustness of the draft sample handling and storage protocol.Methods Samples of blood and urine were collected from 40 healthy volunteers and either processed immediately according to the protocol or maintained at specified temperatures (4 degrees C for all tubes with the exception of vacutainers containing acid citrate dextrose that were maintained at 18 degrees C) for 12, 24 or 36 h prior to processing. A further sample was maintained for 24h at 4 degrees C, processed and the aliquots frozen at -80 degrees C for 20 days and then thawed under controlled conditions. The stability of the samples was compared for the different times in a wide variety of assays.Results The samples maintained at 4 degrees C were stable for at least 24h after collection for a wide range of assays. Small but significant changes were observed in metabonomic studies in samples maintained at 4 degrees C for 36h. There was no degradation of the samples for a range of biochemical assays after short-term freezing and thawing under controlled conditions. Whole blood maintained at 18 degrees C for 24h in vacutainers containing acid citrate dextrose is suitable for viral immortalization techniques.Conclusions The validation studies reported in this supplement provide justification for the sample handling and storage procedures adopted in the UK Biobank project.