Circulating tumor cell identification by functionalized silver-gold nanorods with multicolor, super-enhanced SERS and photothermal resonances.
Circulating tumor cell identification by functionalized silver-gold nanorods with multicolor, super-enhanced SERS and photothermal resonances.
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DOI：
10.1038/srep04752
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发表时间：
2014-05-09
影响因子：
4.6
通讯作者：
Biris AS
中科院分区：
文献类型：
--
作者：
Nima ZA;Mahmood M;Xu Y;Mustafa T;Watanabe F;Nedosekin DA;Juratli MA;Fahmi T;Galanzha EI;Nolan JP;Basnakian AG;Zharov VP;Biris AS
纳米技术已被广泛探索用于癌症诊断。然而，由于生物偶联纳米颗粒的颜色重叠，目前同时识别几个癌症生物标志物的方法的特异性有限。在这里，我们提出了一种技术，通过使用具有窄表面增强拉曼散射(SERS)和高光热对比度的可调银-金纳米棒来提高癌症诊断的分子和光谱特异性。银金纳米棒被四个拉曼活性分子和四个针对乳腺癌标记物和白细胞特异性CD45标记物的抗体功能化。与传统的金纳米棒相比，这些混合纳米系统的SERS信号增强了两个数量级以上。使用抗体彩虹鸡尾酒，我们展示了在未经处理的人血中检测单个乳腺癌细胞的高度特异性。通过集成多路靶向、多色编码和多模式检测，我们的方法具有改善复杂生物环境中单个肿瘤细胞的多光谱成像的潜力。
Nanotechnology has been extensively explored for cancer diagnostics. However, the specificity of current methods to identify simultaneously several cancer biomarkers is limited due to color overlapping of bio-conjugated nanoparticles. Here, we present a technique to increase both the molecular and spectral specificity of cancer diagnosis by using tunable silver-gold nanorods with narrow surface-enhanced Raman scattering (SERS) and high photothermal contrast. The silver-gold nanorods were functionalized with four Raman-active molecules and four antibodies specific to breast cancer markers and with leukocyte-specific CD45 marker. More than two orders of magnitude of SERS signal enhancement was observed from these hybrid nanosystems compared to conventional gold nanorods. Using an antibody rainbow cocktail, we demonstrated highly specific detection of single breast cancer cells in unprocessed human blood. By integrating multiplex targeting, multicolor coding, and multimodal detection, our approach has the potential to improve multispectral imaging of individual tumor cells in complex biological environments.
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影响因子：
29.4
作者：
Jana, Nikhil R.;Pal, Tarasankar
通讯作者：
Pal, Tarasankar
影响因子：
4.8
作者：
Nolan, John P.;Duggan, Erika;Liu, Er;Condello, Danilo;Dave, Isha;Stoner, Samuel A.
通讯作者：
Stoner, Samuel A.
影响因子：
17.1
作者：
Ozkumur E;Shah AM;Ciciliano JC;Emmink BL;Miyamoto DT;Brachtel E;Yu M;Chen PI;Morgan B;Trautwein J;Kimura A;Sengupta S;Stott SL;Karabacak NM;Barber TA;Walsh JR;Smith K;Spuhler PS;Sullivan JP;Lee RJ;Ting DT;Luo X;Shaw AT;Bardia A;Sequist LV;Louis DN;Maheswaran S;Kapur R;Haber DA;Toner M
通讯作者：
Toner M
影响因子：
8.8
作者：
通讯作者：
--
影响因子：
56.9
作者：
Gaiduk, A.;Yorulmaz, M.;Orrit, M.
通讯作者：
Orrit, M.