Fluorescence fluctuation spectroscopy: ushering in a new age of enlightenment for cellular dynamics.
Fluorescence fluctuation spectroscopy: ushering in a new age of enlightenment for cellular dynamics.
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DOI：
10.1007/s12551-009-0013-8
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发表时间：
2009-09-01
影响因子：
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通讯作者：
Albanesi, Joseph P
中科院分区：
文献类型：
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作者：
Jameson, David M;Ross, Justin A;Albanesi, Joseph P
荧光涨落光谱最初是为了在物理和物理化学中的应用而开发的，现在正在细胞生物学中得到广泛的应用。本文回顾了该方法的发展，并描述了一些更重要的应用。具体来说，讨论的方法包括荧光相关光谱(FCS)、扫描FCS、双色互相关FCS、光子计数直方图和荧光强度分布分析方法、光栅扫描图像相关光谱方法以及数字和亮度技术。这些方法背后的物理原理将被描绘出来，每种方法都将用文献中的例子进行说明。
Originally developed for applications in physics and physical chemistry, fluorescence fluctuation spectroscopy is becoming widely used in cell biology. This review traces the development of the method and describes some of the more important applications. Specifically, the methods discussed include fluorescence correlation spectroscopy (FCS), scanning FCS, dual color cross-correlation FCS, the photon counting histogram and fluorescence intensity distribution analysis approaches, the raster scanning image correlation spectroscopy method, and the Number and Brightness technique. The physical principles underlying these approaches will be delineated, and each of the methods will be illustrated using examples from the literature.