The superficial layers of the superior colliculus are cytoarchitectually and myeloarchitectually disorganized in the reelin-deficient mouse, reeler
The superficial layers of the superior colliculus are cytoarchitectually and myeloarchitectually disorganized in the reelin-deficient mouse, reeler
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DOI：
10.1016/j.brainres.2006.09.113
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发表时间：
2007-04-06
期刊：
影响因子：
2.9
通讯作者：
Terashima, Toshio
中科院分区：
文献类型：
--
作者：
Baba, Kousuke;Sakakibara, Shunsuke;Terashima, Toshio
reeler小鼠的致病基因是编码Reelin蛋白（一种细胞外分子）的reelin。在本研究中，我们研究了细胞结构，骨髓结构，和传入/传出系统的上级丘（SC）的reeler小鼠。在卷取机中，SC的表面三层的层状结构被打乱并混合成单层，即，如先前在缺乏reelin的SRK大鼠中所报道的（Sakakibara等，开发. Brain Res. 141：1-13）。接下来，我们研究了过程和终端的视觉皮质和retinotectal的预测与注射biocytin到视皮层或注射霍乱毒素亚单位B到视网膜，分别。在reeler，顺行标记的视觉皮质和retinotectal纤维采取了一个异常的过程中的SuF，导致异常的骨髓结构的表面SC的reeler。逆行标记的顶盖脊髓束神经元不能显示任何差异之间的正常和reeler小鼠，这表明深层的reeler SC的cytostructually正常。原位杂交和免疫组织化学研究表明，reelin mRNA和Reelin蛋白都被识别在正常SC。这些结果表明，Reelin蛋白在SC的浅层的组织发生中起着一定的作用。（c）2006 Elsevier B. V.版权所有。
causative gene for the reeler mouse is reelin which encodes Reelin protein, an extracellular molecule. In the present study, we have examined the cytoarchitecture, myeloarchitecture, and afferent/efferent systems of the superior colliculus (SC) of the reeler mouse. In the reeler, the laminar structures of the superficial three layers of the SC were disorganized and intermingled into a single layer, i.e., the superficial fused layer (SuF), as previously reported in the reelin-deficient SRK rat (Sakakibara et al., Develop. Brain Res. 141: 1-13). Next, we have investigated the course and terminals of visual corticotectal and retinotectal projections with an injection of biocytin into the visual cortex or an injection of cholera toxin subunit B into the retina, respectively. In the reeler, anterogradely labeled visual corticotectal and retinotectal fibers took an aberrant course within the SuF, resulting in abnormal myeloarchitecture of the superficial SC of the reeler. Retrograde labeling of tectospinal tract neurons could not show any differences between the normal and reeler mice, suggesting that the deep layers of the reeler SC are cytoarchitectu ally normal. In situ hybridization and immunohistochemical studies have shown that reelin mRNA and Reelin protein were both recognized in the normal SC. These results suggest that Reelin protein plays some roles in histogenesis of the superficial layers of the SC. (c) 2006 Elsevier B.V. All rights reserved.