Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System
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DOI:
10.1104/pp.114.247577
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发表时间:
2014-11-01
期刊:
影响因子:
7.4
通讯作者:
Van Eck, Joyce
Van Eck, Joyce
中科院分区:
生物学1区
文献类型:
--
作者:
Brooks, Christopher;Nekrasov, Vladimir;Van Eck, Joyce

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CRISPR/Cas9系统在稳定的转基因番茄植物中产生靶向突变方面非常有效,并且可以在第一代中产生所需大小的纯合缺失。
The CRISPR/Cas9 system is highly efficient at generating targeted mutations in stable transgenic tomato plants, and homozygous deletions of a desired size can be created in the first generation.